伪协约的卡伊尼 诺维亚,西班牙. nov.,从活性污泥中分离出来
Na Li1,2, Yingxin Yang1, Jing Wu1
1College of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang, Henan 473061, PR China.
概括
从活性污泥中确定了一种新型细菌属和物种Pseudokordiimonas caeni. 这一发现扩大了Kordiimonadaceae家族内已知的多样性.
科学领域:
- 微生物学 微生物学
- 细菌的分类学 细菌的分类学
- 环境微生物学 环境微生物学
背景情况:
- 活性污泥含有各种微生物群落,对废水处理至关重要.
- 科尔迪蒙纳家族 (Kordiimonadaceae) 包括具有各种代谢能力的细菌.
- 鉴定新型细菌分离物的特征对于了解微生物生态系统至关重要.
研究的目的:
- 从活性污泥中分离和描述一种新型细菌菌株.
- 确定新型隔离物在Kordiimonadaceae家族中的分类地位.
- 为独特的细菌分离物提出一个新的属和物种.
主要方法:
- 从活性污泥中分离和培养细菌菌株LB-31T .
- 现型特征包括Gram染色,形态和生长条件 (温度,pH,盐度).
- 基因组分析包括基因组测序,G+C含量确定和基于16S rRNA基因序列的遗传学分析.
- 基因组比较分析包括平均核酸标识 (ANI) 和数字DNA-DNA杂交 (dDDH).
- 化学分类学分析包括呼吸,脂肪酸概况和极性脂质组成.
主要成果:
- 菌株LB-31T是一种格拉姆阴性,不形成子的杆子,具有单个极性鞭毛体,在介质性条件下,中性pH值和中等盐度下呈现最佳生长.
- 遗传学分析显示,LB-31T菌株代表Kordiimonadaceae家族中的一个独特的血统,16S rRNA基因序列相似性低于已知物种的94%.
- 基因组和化学分类学数据,包括与其最近的亲属的低ANI (<72%) 和dDDH (<22%) 值,证实了其作为新品种和新品种的地位.
- 主要的呼吸是Q-10,具有特征性的脂肪酸特征和主要的极性脂质,包括酸乙醇胺,二酸糖醇和酸糖醇.
结论:
- 菌株LB-31T代表Kordiimonadaceae家族中一个新型属的新型物种.
- 这种新型分类的建议名称是 *Pseudokordiimonas caeni* gen. nov., sp. 作为类型菌株的 LB-31T 菌株.
- 这一发现有助于了解活性污泥中的细菌多样性,并扩大了Kordiimonadaceae家族.
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