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Microtubules are thick hollow cylindrical proteins that help form the cytoskeleton. Microtubules have varied roles in the cell. These filaments help form cellular appendages like cilia and flagella, which are responsible for locomotion. The cilia arise from basal bodies, separated from the main body by a membrane-like structure forming the transition zone. This zone is the gate for the entry of lipids and proteins, creating a unique composition of lipids and proteins in the ciliary membrane and...
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The ciliary structures were first seen in 1647 by Antonie Leeuwenhoek while observing the protozoans. In lower organisms, these appendages are responsible for cell movement, while in higher organisms, these appendages help in the movement of the extracellular fluids within the body cavities.
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基于光激活的微管的二维活跃阴影.

Zahra Zarei1, John Berezney1, Alexander Hensley1

  • 1The Martin Fisher School of Physics, Brandeis University, Waltham, Massachusetts 02454, USA. fraden@brandeis.edu.

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概括
此摘要是机器生成的。

与流程式opto-K401电机相比,非流程式opto-K365电机显著增强光驱动的微管子活体阴性流. 这项研究揭示了缺陷密度与流量之间的明显的短暂动态,使得能够对活性物质进行有针对性的控制.

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科学领域:

  • 生物物理学的生物物理.
  • 软物质物理学 软物质物理学
  • 活动物质物理学 活动物质物理学

背景情况:

  • 基于微管的活体体质是由分子电机驱动的动态系统.
  • 响应光的电机提供了对活性物质动态的外部控制.
  • 了解运动性质 (进程性) 是控制活跃的阴性行为的关键.

研究的目的:

  • 为了比较两种光响应性激素电机 (opto-K401,过程性;opto-K365,非过程性) 在驱动微管子活体内马的效率.
  • 为了描述流量和缺陷动态,以应对不同的光强度.
  • 为了研究不同活动状态之间的过渡期间的短暂行为.

主要方法:

  • 使用响应光的激素电机集群 (opto-K401和opto-K365) 来产生微管子活体体质.
  • 使用显微镜在受控照明下测量阴性流速和缺陷密度.
  • 分析对光强度变化的稳定状态和短暂反应.

主要成果:

  • 与opto-K401.1相比,opto-K365电机在高光和低光条件之间的内马式流速中显示出一个数量级更大的对比度.
  • 稳定状态流量和缺陷密度的特征是光强度对opto-K365 nematics的函数.
  • 与流动动力学 (几十秒钟) 相比,缺陷密度表现出较慢的短暂响应 (长达10分钟).

结论:

  • 非进程式opto-K365电机在光控制的活力内马特动力学方面比进程式opto-K401电机更有效.
  • 活跃流动和结构状态恢复的时间尺度之间存在分离.
  • 这项工作为利用时空控制在活性物质中产生有针对性的动态状态提供了一个平台.