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婴儿白杆菌 (Bifidobacterium infantis) 使用含有N-乙葡萄糖胺的人类牛奶寡糖体作为源

Shuqi Li¹, Xiaomeng You¹, Asha Rani¹

¹Department of Food Science, University of Massachusetts, Amherst, MA, USA.

|August 23, 2023

概括

双细菌婴儿使用来自人乳寡糖的,特别是N-乙葡萄糖胺 (NAG). 这项研究详细介绍了B. infantis如何代谢HMO,影响婴儿肠道健康研究.

科学领域:

微生物学微生物学
人类牛奶寡糖 (HMO) 代谢
肠道微生物组研究研究

背景情况:

双细菌长子物种双菌长子物种婴儿 (B. infantis) 是婴儿的关键肠道开始体.
人类母乳寡糖化物 (HMO) 是婴儿肠道细菌的关键营养素.
B. infantis从HMO中的利用,特别是N-乙糖胺 (NAG),仍然在很大程度上没有特征.

研究的目的:

调查B. infantis从含有特定N-乙糖胺 (NAG) 的母乳寡糖化物 (HMO) 的利用能力.
阐明B. infantis.参与HMO化所涉及的代谢途径和调节网络.

主要方法:

在体外生长动力学实验中使用LNT和LNnT作为源.
稳定同位素标记15N-NAG以跟踪的纳入蛋白质组.
测量细胞内二氧格酸盐水平.
进行比较的转录组学和蛋白组学分析.

主要成果:

B. infantis 证明了从LNT和LNnT中利用的能力.
同位素标记的NAG被纳入B. infantis蛋白质中.
在HMO中的利用导致了细胞内2-oxoglutarate水平的调节.
转录基因和蛋白质基因数据揭示了在HMO化过程中激活的特定调节网络.

结论:

B. infantis 拥有一个功能系统,可以利用含有 N-乙糖胺 (NAG) 的特定 HMO 的.
这种利用影响了细菌的新陈代谢和基因表达,突出了新的营养策略.
这些发现对理解婴儿肠道殖民和开发向益生菌或益生菌有意义.

关键词:

这就是2-oxoglutarate.双杆菌 (Bifidobacteria) 是一种细菌,它可以人类牛奶中的寡糖类.我们的微生物群.的新陈代谢的新陈代谢

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