颗粒和细胞在连续流动中进行电介质组装和分离
Xiaoming Chen1,2, Shun Liu1,2, Mo Shen1,2
1School of Control Engineering, Northeastern University at Qinhuangdao, Qinhuangdao 066004, PR China. chenxiaoming@neuq.edu.cn.
Analytical methods : advancing methods and applications
|August 23, 2023
概括
这项研究开发了一种微流体装置,用于二极电泳 (DEP) 分离,改善细胞和粒子的隔离. 该设备有效地组装和分离各种粒子和细胞,展示了针对性隔离的高纯度率.
科学领域:
- 生物物理学的生物物理.
- 微流体学 微流体学
- 生物技术是生物技术.
背景情况:
- 在临床,制药和环境应用中,介电离分离 (DEP) 是至关重要的.
- 了解DEP力下的粒子和细胞组装是优化分离效率的关键.
- 现有的方法需要进一步开发,以实现大规模,高效的隔离.
研究的目的:
- 通过使用具有双极电极阵列的新型微流体装置,研究粒子和细胞组装和分离机制.
- 分析珍珠链形成对分离纯度和效率的影响.
- 为了证明持续的,高效的目标细胞和颗粒的隔离.
主要方法:
- 开发了一种具有双极电极阵列的微流体装置.
- 在交流电场下的单粒子和多粒子组件的观察和分析.
- 流速的系统变化和对聚乙烯颗粒和微藻 (酵母,Scenedesmus sp.,C. vulgaris,Oocystis sp. 酵母菌) 的分离纯度的分析. ) 的情况.
主要成果:
- 高纯度率 (>94.9%) 实现了5μm聚乙烯颗粒的高纯度,并优化了流速.
- 细胞链形成对C. vulgaris.隔离效率的证明影响.
- 当Oocystis sp.观察到C. vulgaris的度下降时 链条形成,而纯度保持不变.
结论:
- 开发的微流体DEP装置使粒子和细胞的高效大规模组装和分离成为可能.
- 珍珠链形成显著影响分离动态,需要仔细考虑优化.
- 这项技术为高效隔离目标生物实体提供了一个有前途的平台.
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