NRON和TUG1长非编码RNA在炎症和EAE病变发生过程中的参与
Emad Kioumarsi1, Leila Kohan1, Farshid Noorbakhsh2
1Department of Biology, Arsanjan Branch, Islamic Azad University, Arsanjan, Iran.
Nucleosides, nucleotides & nucleic acids
|August 23, 2023
概括
在多发性硬化症 (MS) 模型中发现长非编码RNAs核抑制NFAT (NRON) 和氨酸上调调1 (TUG1) 的下调. 这些lncRNAs与炎症性细胞因子呈负相关性,这表明它们在MS的发病过程中起作用.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 长非编码RNAs (lncRNAs) 越来越多地与多发性硬化症 (MS) 等神经退行性疾病有关.
- 在MS病变发生过程中,特别是炎症过程中, lncRNAs核抑制NFAT (NRON) 和氨酸上调1 (TUG1) 在MS病变发生过程中的特定作用仍未得到充分研究.
研究的目的:
- 为了研究NRON和TUG1在实验性自身免疫脑膜炎 (EAE) 的急性和慢性阶段的表达模式,一个MS模型.
- 检查NRON和TUG1表达和EAE中关键炎症性细胞因子之间的相关性.
- 在炎症条件下评估NRON和TUG1在培养的巨细胞中的表达.
主要方法:
- 在C57BL/6小鼠中使用MOG35-55/CFA诱导EAE模型.
- 对临床评分的评估和在28天内牺牲小鼠进行组织采集.
- 使用实时RT-PCR对脊髓组织和培养的巨细胞中NRON,TUG1,IL-1,IL-6和CDe-3表达的量化.
主要成果:
- 与对照组相比,NRON和TUG1表达在EAE急性阶段在腰椎脊髓中显著下调.
- 与对照巨相比,NRON和TUG1在用脂聚糖 (LPS) 治疗的巨中也被显著降低.
- 观察到NRON和TUG1表达水平与炎症性细胞因子IL-1,IL-6和CDe-3的表达之间存在负相关性.
结论:
- 该研究表明,在EAE模型的脊髓病变和激活的巨细胞中,lncRNAs TUG1和NRON的显著失调.
- 这些发现表明NRON和TUG1可能在MS的炎症病原发生中发挥关键作用.
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