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诱导后人类诱导的多能干细胞培养和表征的协议

Yu-Shan Cheng¹, Miao Xu¹, Guibin Chen¹

¹National Center for Advancing Translational Sciences, National Institutes of Health, Bethesda, Maryland.

Current protocols

|August 23, 2023

概括

这项研究提供了培养和表征人类诱导多能干细胞 (hiPSCs) 的详细方案. 这些方法确保高质量的hiPSC用于疾病建模和再生医学应用.

科学领域:

干细胞生物学干细胞生物学
再生医学是一种再生医学.
细胞生物学细胞生物学

背景情况:

人类诱导的多能干细胞 (hiPSCs) 具有自我更新和分化能力.
hiPSCs对于疾病建模,药物开发,细胞疗法和再生医学具有价值.
维持高质量的hiPSCs需要特殊的技术,与标准细胞系培养不同.

研究的目的:

为hiPSC传播提供全面,逐步的协议.
详细介绍hiPSC表征和质量控制的方法.
作为与hiPSCs工作的实验室工作人员的参考指南.

主要方法:

化学定义,无料培养条件用于hiPSC的繁殖.
免疫细胞化学和流动细胞计分析用于多能性标志物的验证.
在体内测试瘤形成试验,以评估多能性.
RT-PCR用于确认仙台病毒载体清除.

主要成果:

建立了成功hiPSC扩张和传播的协议.
评估hiPSC多能性和质量的详细方法.
在培养后确保消除病毒载体.

结论:

提供的协议使得高质量hiPSC的持续生成成为可能.
这些方法对于研究和治疗中可靠的下游应用至关重要.
本指南支持实验室工作人员掌握hiPSC培养技术.

关键词:

通过 hiPSC 的表征.这就是hiPSC冷保存.在 hiPSC 文化中通过 hiPSC 的通道.人类诱导的多能干细胞干细胞.一个特拉托马体.

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