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Viral Recombination

Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.

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相关实验视频

Updated: Jul 18, 2025

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins

Published on: April 17, 2020

再组合雪松病毒:一个海尼帕病毒逆转基因平台

Moushimi Amaya¹, Christopher C Broder¹, Eric D Laing²

¹Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA.

Methods in molecular biology (Clifton, N.J.)

|August 23, 2023

概括

研究人员开发了一种新的逆遗传系统,用于Cedar病毒,一种非致病性henipavirus. 该系统使得在生物安全级别-2条件下对尼病毒生物学和病原发生的研究更加安全.

关键词:

雪松树病毒病毒黑尼帕病毒是什么?反向遗传学是一种反向的遗传学.病毒斑块检测试验病毒救援病毒救援病毒定位病毒定位

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Last Updated: Jul 18, 2025

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins

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Published on: January 7, 2019

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科学领域:

病毒学病毒学
分子生物学分子生物学
传染性疾病传染性疾病

背景情况:

雪松病毒是一种非致病性海尼帕病毒,与高度致病性尼帕和亨德拉病毒密切相关.
研究海尼帕病毒通常需要高生物安全制 (BSL-4).
一个非致病性模型为海尼帕病毒研究提供了一个更安全的平台.

研究的目的:

为了建立一个逆转基因系统为Cedar病毒.
为了创建复制能力强的复合雪松病毒变种.
为了实现对病毒病原和抗病毒开发的更安全的研究.

主要方法:

科达病毒的隔离.
开发一种用于病毒救援的逆遗传系统.
一个重组野生类型雪松病毒的生成.
一种表达绿色光蛋白 (GFP) 的复合雪松病毒的生成.

主要成果:

成功地救出了两种复制能力强的重组赛达病毒变种.
对Cedar病毒的功能逆遗传系统的演示.
创建一个表达GFP的Cedar病毒用于跟踪和操纵.

结论:

开发的逆转基因系统为海尼帕病毒研究提供了有价值的工具.
在生物安全级别-2条件下,可以安全地研究复合雪松病毒.
这个平台有助于对henipavirus的发病性,受体热带性和抗病毒发现的研究.