乙型肝炎病毒包裹抗原和乙型肝炎病毒表面抗原都会在持续的乙型肝炎病毒感染期间促进先天免疫反应
Jie-Min Zhang1, Na-Ling Kang2, Lu-Ying Wu2
1Department of Pharmacy, The First Affiliated Hospital, Fujian Medical University, Fuzhou, China.
Viral immunology
|August 23, 2023
概括
持续性乙型肝炎病毒 (HBV) 感染通过减少类似收费受体4 (TLR4) 和自然杀手 (NK) 细胞,同时增加T细胞和单细胞,从而改变先天免疫力. 乙型肝炎表面抗原 (HBsAg) 和包膜抗原 (HBeAg) 显示出类似的效果.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 肝病学 肝病学是一种肝病学.
背景情况:
- 持续性乙型肝炎病毒 (HBV) 感染对全球健康构成重大挑战.
- 了解慢性HBV感染期间的先天免疫反应对于开发有效疗法至关重要.
- 收费类受体4 (TLR4) 和细胞因子表达是先天免疫的关键组成部分.
研究的目的:
- 在小鼠模型中研究持续性HBV感染对TLR4表达,促炎细胞因子和先天性免疫细胞群的影响.
- 为了区分乙型肝炎病毒感染,乙型肝炎表面抗原 (HBsAg) 和乙型肝炎包膜抗原 (HBeAg) 对天生的免疫系统的影响.
主要方法:
- 使用复合腺相关病毒开发持续HBV,HBsAg和HBeAg表达的小鼠模型.
- 与酶相关的免疫吸收试验 (ELISA) 检测血清细胞因子水平.
- 免疫组织化学和西部斑对于TLR4表达.
- 流式细胞计量用于天生的免疫细胞百分比.
主要成果:
- 持久的HBV,HBsAg和HBeAg显著降低了TLR4的表达.
- 乙型肝炎病毒感染增加了T细胞和单细胞,同时减少了自然杀手细胞 (NK).
- HBeAg减少了NK细胞,HBsAg增加了NK细胞,两者都增加了单细胞但减少了T细胞.
结论:
- 持续的HBV感染,HBsAg和HBeAg显著调节先天免疫反应,包括TLR4表达和免疫细胞组成.
- 特定的病毒抗原 (HBsAg和HBeAg) 对免疫细胞百分比有明显但重叠的影响.
- 这些发现提供了关于HBV和慢性感染期间天生的免疫系统之间的复杂相互作用的见解.
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