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对花突变物进行的比较转录组分析和黄类样本分析揭示了CmMYB11调节香花的黄类生物合成

Yiguang Wang¹, Li-Jie Zhou¹, Aiping Song¹

¹State Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, College of Horticulture, Nanjing Agricultural University, 210095 Nanjing, China.

Plant science : an international journal of experimental plant biology

|August 23, 2023

概括

花的花色突变揭示了黄类生物合成的关键调节者. 降低CmMYB11的调节会影响全方位的黄,而CmMYB6和CmbHLH2会影响素水平,从而提供了植物色彩的洞察力.

科学领域:

植物生物学植物生物学
分子遗传学分子遗传学
生物化学生物化学

背景情况:

黄类化合物,包括氨酸,对于植物的色彩至关重要.
花花的花蕾体育表现出因遗传突变而导致的花颜色变化.
在突变花中控制着类生物合成的调节网络在很大程度上仍然没有被描述.

研究的目的:

为了研究花花的花色突变体中黄类生物合成的调节机制.
确定涉及改变色素生产的关键转录因子.
了解花种类中颜色变化的遗传基础.

主要方法:

对花品种及其花芽体育中的表型,色素组成和转录体的比较分析.
对黄生物合成和调节基因 (MYB,bHLH) 的差异基因表达分析.
在烟草中进行促进体活性测定和异质表达,以验证基因功能.

主要成果:

"阿纳斯塔西亚深绿"和"阿纳斯塔西亚粉红"花品种的突变导致了酸盐和总黄酸盐含量的改变.
观察到多个类黄生物合成基因和转录因子 (CmMYB6,CmbHLH2,CmMYB11) 的差异表达.
CmMYB11被确定为几个黄类生物合成基因的直接激活剂,并在烟草中过度表达时增加了黄醇和氨酸.

结论:

CmMYB11在调节花中的黄醇和酸生物合成方面发挥着重要作用.
CmMYB6和CmbHLH2与花色突变的花色色素含量变化相关.
这项研究阐明了花黄类生物合成和着色中的新型调节网络.

关键词:

花花 (Chrysanthemum) 是一种花.在CmMYB11中使用.黄类生物合成鲜花的颜色鲜花的颜色变种突变者变种突变者转录因子转录因子转录组的概况分析

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