维布里奥 (Vibrio cyclitrophicus) 在海洋足动物上形成种群特异的生物膜和生长
Ryan A Nuttall1, Pia H Moisander1
1Department of Biology, University of Massachusetts Dartmouth, North Dartmouth, Massachusetts, USA.
Environmental microbiology
|August 23, 2023
概括
来自copepods和海水的Vibrio cyclitrophicus菌株表现出不同的能力来形成生物膜并附着在copepods上. 影响了这种殖民,揭示了海洋Vibrio-copepod协会中的独特机制.
科学领域:
- 海洋微生物学 海洋微生物学
- 微生物生态学 微生物生态学
- 动物浮游生物与微生物的相互作用
背景情况:
- 维布里奥病毒 spp. 是足动物微生物组的一部分,但这种协会的生物机制尚不清楚.
- 足动物是海上丰富的平原生物,使它们的微生物相互作用在生态上具有重要意义.
研究的目的:
- 为了比较由Vibrio cyclitrophicus从copepods (L-菌株) 和海水 (S-菌株) 中分离的菌株的生物膜形成和殖民.
- 调查盐成分,特别是在Vibrio生物膜形成和附着于copepods中的作用.
主要方法:
- 在不同盐的条件下 (完整的海盐,NaCl,NaCl +) 对生物膜形成的比较分析.
- 视觉化和量化Vibrio cyclitrophicus (标记为GFP的L菌株) 在活足 (A. tonsa) 上的附着和生长.
- L菌株和S菌株的遗传比较 (平均核酸标识).
主要成果:
- 在完整的海盐中,S和L菌株都形成了生物膜,这表明S菌株中存在新的生物膜机制.
- 显著增强了L菌株的生物膜形成,而单独的NaCl并不支持生物膜的形成.
- 与S菌株相比,L菌株的细胞对活足的附着数量较高,这表明涉及的不同殖民机制.
- 维布里奥循环的细胞在活的copepods上表现出生长,证实了copepods作为生生长的基质.
结论:
- 尽管具有很高的遗传相似性 (99.1% ANI),但Vibrio cyclitrophicus菌株在殖民海洋足动物方面表现出不同的能力.
- 独特的机制,可能涉及,通过特定的Vibrio菌株来调解copepod的殖民.
- 维布里奥循环 - 足动物 (A. tonsa) 模型系统为未来关于维布里奥-足动物关联的研究提供了一个有价值的平台.
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