使用多核纤维捆绑的多模态非线性内镜的可行性研究,用于从组织部分到散装器官的远程扫描
Hyeonsoo Bae1,2,3, Marko Rodewald1,2, Tobias Meyer-Zedler1,2
1Leibniz Institute of Photonic Technology (Leibniz IPHT), Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research (LPI), PO Box 100239, 07702, Jena, Germany.
Scientific reports
|August 23, 2023
概括
一个新的多核光纤探测器可以实现无标签的多模式非线性成像. 这种紧的探头为先进的生物医学成像应用提供了更好的临床兼容性.
科学领域:
- 生物医学光学 生物医学光学
- 先进的成像技术 技术的成像技术
- 光纤探测器 光纤探测器
背景情况:
- 多模式非线性成像为生物组织提供丰富的对比度.
- 由于移动的部件,现有的光纤探头往往不符合临床要求.
- 无标签成像模式对于最小化样本扰动至关重要.
研究的目的:
- 开发和评估一个紧的多核光纤探测器,用于多式模式的非线性成像.
- 评估探头与临床要求的兼容性.
- 在临床相关的组织样本上证明探针的性能.
主要方法:
- 该探测器集成了连贯抗斯托克斯拉曼散射 (CARS),第二波生成 (SHG) 和双光子激发光 (TPEF).
- 使用冷切割和人工目标验证了性能.
- 活体人类和猪肠道组织被用于临床适用性评估.
- 使用图像重建算法来处理像素化数据.
主要成果:
- 纤维探针设计消除了远端的移动和带电压的部件,提高了临床兼容性.
- 在ex vivo肠道组织上实现了高图像质量和形态化学一致性.
- 探测器的性能与传统的激光扫描显微镜相美.
- 一个简单的重建程序产生了令人满意的结果.
结论:
- 开发的多核光纤探头是无标签多式多态非线性成像的一个有希望的工具.
- 探针的设计为临床翻译提供了显著的优势.
- 为现实世界的临床评估概述了进一步发展的途径.
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