免疫景观和HPV相关结直肠癌中的菌生物:一项探索性研究
Maria Raffaella Ambrosio1, Elena Niccolai2, Federica Petrelli1
1Pathology Unit, Azienda USL Toscana Nord Ovest, Pisa, Italy.
Clinical and experimental medicine
|August 23, 2023
概括
人类乳头瘤病毒 (HPV) 感染与20-40%的结直肠癌 (CRC) 有关,影响瘤发育和免疫反应. 阳性CRC呈现出不同的微生物群和改变的瘤微环境.
科学领域:
- 在瘤学瘤学.
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
背景情况:
- 与人类乳头瘤病毒 (HPV) 相关的恶性瘤影响全球超过55万名患者.
- 只有很小一部分感染HPV的个体会患上癌症,这表明还有其他因素导致癌症.
- HPV在结直肠癌 (CRC) 发展中的作用尚不清楚,研究结果相互矛盾.
研究的目的:
- 在结直肠癌 (CRC) 样本中调查HPV感染.
- 分析HPV对瘤微环境和免疫细胞表型的影响.
- 为了比较HPV阳性与HPV阴性CRC的肠道微生物组成.
主要方法:
- 免疫组织化学 (IHC),实时PCR和RNA测序用于检测50名CRC患者的HPV.
- 瘤微环境分析在瘤和非瘤组织上进行.
- 使用16S rRNA测序研究了与CRC相关的微生物群.
主要成果:
- 在20%的右侧CRC和40%的左侧CRC中检测到HPV感染.
- 阳性CRC没有p53和RB的表达,这表明HPV在瘤发生中的作用.
- 与HPV相关的癌症显示免疫监测减少,但增加了淋巴细胞细胞毒性反应.
- 在HPV阳性与HPV阴性CRC中观察到不同的微生物群概况.
结论:
- 在结直肠癌的发展和瘤发生过程中,HPV起着重要作用.
- 在CRC中,HPV会影响瘤免疫微环境.
- 感染HPV与CRC相关微生物群的特定变化有关.
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