一种3D打印的多隔间装置,可实现抗生素PK/PD的动态分析
Andrew A Heller1,2, Morgan K Geiger2, Dana M Spence3,4,5
1Department of Chemistry, Michigan State University, East Lansing, MI, 48824, USA.
Analytical and bioanalytical chemistry
|August 23, 2023
概括
研究人员开发了一个3D打印的两模型,以更好地模拟活体中的抗生素药理动力学 (PK). 这种动态模型准确地预测了抗生素对抗耐药细菌的活性,改善了药物开发.
科学领域:
- 生物医学工程 生物医学工程
- 药理学 药理学是指药理学的学科.
- 微生物学 微生物学
背景情况:
- 抗生素耐药性是一个日益增长的威胁,由于缺乏新药开发而加剧.
- 目前的抗生素敏感性测试在体内缺乏药物动力学意义.
- 静态敏感性测试不能准确地反映药物如何在体内表现.
研究的目的:
- 设计和验证一种新的3D打印的两模型,用于模拟体内药理动力学.
- 评估该模型在评估抗生素对抗耐药细菌菌株的活性时的有用性.
- 为早期抗生素开发提供一个更现实的平台.
主要方法:
- 一个3D打印设备的制造,配有立方插件,具有分子扩散膜.
- 使用光素模拟药物从血扩散到间歇液体的模型的表征.
- 测试动态模型与二次区中的グラム阳性和グラム阴性细菌,测量抗生素活性 (ATP/OD600).
主要成果:
- 3D打印模型产生了与已公布的体内数据可比的药代动力学 (PK) 值.
- 该模型成功地证明了抗生素活性对抗抗性大肠杆菌和B. subtilis菌株的抗生素活性.
- 早在3小时后就发现了抗生素活性的统计学显著差异,与特定药物度相关.
结论:
- 制造的两模型准确地模仿了人类的药理动力学.
- 这种动态模型对于确定抗生素药理动力学是有效的.
- 该平台为推进抗生素研发提供了一个有价值的工具.
相关概念视频
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