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基于综合生物信息学和单细胞RNA-seq分析,探索系统性红斑狼和初级Sjögren综合征之间的共享分子机制

Yanling Cui^1,2, Huina Zhang^1,2, Zhen Wang^1,2

¹Stem Cell Translational Research Center, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.

Frontiers in immunology

|August 24, 2023

概括

这项研究确定了共同的基因 (IFI44L,ISG15,ITGB2) 和转录因子 (STAT1,IRF7) 在全身性红斑狼 (SLE) 和原发性Sjögren常见.

科学领域:

免疫学和风湿病学
基因组学和生物信息学
分子生物学分子生物学

背景情况:

系统性红斑狼 (SLE) 和原发性Sjögren综合征 (pSS) 是系统性自身免疫性疾病,具有重叠的临床和血清学特征.
了解共享的遗传和分子通路对于阐明常见的病变发生至关重要.
以前的研究还没有完全探索SLE和pSS之间基因表达和信号通路的共同点.

研究的目的:

为了确定SLE和pSS之间的共享基因,信号通路和转录因子 (TFs).
阐明这两种自身免疫性疾病背后的常见病原遗传机制.
为进一步对共同治疗目标的机制研究提供基础.

主要方法:

从SLE和pSS患者的基因表达特征分析使用权重基因共同表达网络分析 (WGCNA) 和差异性基因表达 (DEG) 分析.
重叠的基因受到基因本体学 (GO) 和蛋白质-蛋白质相互作用 (PPI) 网络分析.
使用Cytoscape插件识别了共享的枢纽基因和TF,并通过独立的微阵列和单细胞RNA测序 (scRNA-seq) 数据集进行验证.

主要成果:

确定了152个共享基因,主要涉及干扰素 (IFN) 反应和细胞因子介导的信号传递.
选了六种共享基因,IFI44L,ISG15和ITGB2被证实在两种疾病中都高度表达.
确定STAT1和IRF7是常见的TF,并强调IFN响应和ITGB2信号通路是关键的.

结论:

IFI44L,ISG15和ITGB2被确定为关键的共享基因,而STAT1和IRF7是SLE和pSS中常见的TF.
干扰素反应和ITGB2信号通路在两种疾病的发病过程中都起着重要作用.
这项研究揭示了常见的病原遗传特征,为未来的研究和治疗策略提供了潜在的目标.

关键词:

在TFs中,我们可以使用TFs.生物信息学是一种生物信息学.核心基因核心基因核心基因主要的Sjögren综合征是什么这就是 scRNA-seqq.系统性红血性狼 (Systemic Lupus Erythematosus) 是一种全身性狼.

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