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日本 (Humulus japonicus) 通过抑制小鼠的NF-κB p65依赖性炎症反应来缓解刺激性接触性皮肤炎

Yu-Bin Kim^1,2, Eun-Jung Kang¹, Jung-Ran Noh¹

¹Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea.

Experimental and therapeutic medicine

|August 24, 2023

概括

日本 (Humulus japonicus) 提取物及其化合物黄素在刺激性接触性皮炎 (ICD) 模型中降低皮肤炎症. 这种草药抑制了关键的炎症通路,为ICD提供了有前途的治疗方法.

科学领域:

皮肤病学皮肤病学
药理学药理学是指药理学的学科.
自然产品自然产品

背景情况:

刺激性接触性皮肤炎 (ICD) 是一种由外部刺激物引起的急性炎症性皮肤疾病.
传统草药Humulus japonicus (HJ) 具有已知的抗炎,抗微生物和抗氧化特性.

研究的目的:

研究Humulus japonicus (HJ) 对刺激性接触性皮肤炎 (ICD) 的抗皮炎作用.
阐明HJ在ICD治疗中的治疗潜力的基础分子机制.

主要方法:

使用12-O-tetradecanoylphorbol-13-乙酸盐 (TPA) 诱导的皮肤炎在小鼠和脂多糖 (LPS) 刺激的RAW264.7细胞.
用口服给小鼠的HJ和luteolin和局部TPA诱导皮肤炎.
分析了化学因子 (CCL3,CXCL2),促炎性细胞因子 (IL-1β,IL-6,TNF-α) 和炎症酶 (COX2,iNOS) 的基因表达.
评估的NF-κB p65酸化水平.

主要成果:

在小鼠中,HJ和黄素的使用显著改善了TPA诱导的耳朵红,和免疫细胞透.
在接受治疗的小鼠皮肤中,炎症性化学基因 (CCL3,CXCL2) 和IL-1β的表达减少.
在LPS刺激的RAW264.7细胞中抑制了CCL3,CXCL2,IL-1β,IL-6和TNF-α的基因表达.
减少COX2,iNOS和NF-κB p65酸化的表达,通过HJ和黄素.

结论:

日本 (Humulus japonicus,简称HJ) 显示出对刺激性接触性皮肤炎 (ICD) 的显著保护作用.
肝炎通过抑制关键炎症媒介和通路,包括NF-κB信号传递,发挥其抗皮炎作用.
HJ代表了一种有前途的治疗候选人,用于治疗ICD.

关键词:

12-O-tetradecanoylphorbol-13-乙酸盐的使用情况这种植物叫Humulus japonicus.在 NF-κBB 中.刺激性接触性皮肤炎是一种刺激性皮肤炎.类类类类类类 p6565 这是一个很好的方法.

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