基于生物灵感分子印记聚合物的电化学传感器用于索福斯布维尔检测
Mahmoud A Soliman1, Amr M Mahmoud2, Eman S Elzanfaly2,3
1Misr University for Science and Technology, Faculty of Pharmaceutical Sciences and Drug Manufacturing, Department of Analytical Chemistry 6th of October City 12566 Egypt.
RSC advances
|August 24, 2023
概括
研究人员开发了一种使用基于甲基多巴的分子印记聚合物 (MIP) 的新型传感器,用于高度选择性和敏感地检测索福斯布维尔 (SFB). 这一进步为在制药配方和生物样本中分析SFB提供了可靠的方法.
科学领域:
- 电化学 电化学 电化学
- 聚合物科学 聚合物科学
- 分析化学 分析化学
背景情况:
- 分子印记聚合物 (MIP) 为各种分析物提供了量身定制的识别能力.
- 甲基多巴是一种功能性单体,具有适合创建选择性MIP的多功能化学组.
- 精确检测索福斯布维尔 (SFB) 对于治疗监测和质量控制至关重要.
研究的目的:
- 制造一种选择性MIP传感器,用于检测使用甲基多巴作为功能单体的索福斯布维尔 (SFB).
- 通过实验方法研究甲基多巴和SFB之间的相互作用.
- 评估传感器的性能,包括灵敏度,选择性和在现实世界样本中的适用性.
主要方法:
- 在SFB作为模板的存在下,对甲基多巴在一次性笔石墨电极 (PGE) 上的电聚合.
- 使用X射线光电子光谱 (XPS),电化学阻抗光谱 (ESI) 和循环电压计 (CV) 进行制造的传感器的表征.
- 使用微分脉冲电量计 (DPV) 用铁酸/铁酸氧化还原探针间接检测SFB.
主要成果:
- 开发的MIP传感器在广泛的动态范围 (1.0×10−11 M到1.0×10−13 M) 内显示了可重现和线性响应用于SFB检测.
- 实现了SFB的3.1 × 10-14 M的低检测极限 (LOD).
- 对结构相似的化合物和常见的干扰药物对SFB表现出高选择性.
结论:
- 基于甲基多巴的MIP传感器为SFB检测提供了一个高度敏感和选择性的平台.
- 传感器能够在制药剂量形式和的人类血样本中发挥作用,这突显了其实际实用性.
- 这项研究为准确量化各种矩阵中的SFB提供了有价值的工具.
更多相关视频
08:22Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
Published on: February 16, 2018
12.1K
09:39Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology
Published on: March 31, 2022
3.3K
相关概念视频
03:31Labeling DNA Probes
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
01:17Microbial Biosensors
Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...