在小麦半变化过程中,DMC1在高温和低温下稳定交叉
Tracie N Draeger1, María-Dolores Rey2, Sadiye Hayta1
1John Innes Centre, Norwich Research Park, Norwich, United Kingdom.
Frontiers in plant science
|August 24, 2023
概括
小麦的生育能力依赖于染色体突触和交叉,它们对温度敏感. 基因TaDMC1-D1对于维持这些过程在不同温度下至关重要,影响小麦产量.
科学领域:
- 植物遗传学 植物遗传学
- 分子生物学分子生物学
- 生殖生物学 生殖生物学
背景情况:
- 染色体突触和交叉形成对于小麦的生育至关重要.
- 这些介质过程对温度敏感,其最佳功能在17-23°C之间.
- 在小麦半变化中这种温度敏感性的遗传基础在很大程度上仍然未知.
研究的目的:
- 研究TaDMC1-D1在依赖温度的染色体突触和小麦交叉形成中的作用.
- 在小麦中创建和表征CRISPR-Cas9针对DMC1的向突变.
- 确认TaDMC1-D1在不同温度下保持介质完整性的功能.
主要方法:
- 在小麦品种"中国春天"中开发了一种CRISPR-Cas9向突变 (CRISPR dmc1-D1).
- 控制环境实验暴露野生类型和突变小麦系 (CRISPR dmc1-D1和ttmei1) 介质变异过程中的低 (13°C) 和高 (30°C) 温度.
- 在受治疗的植物中分析染色体突触和交叉形成.
主要成果:
- 与野生型相比,CRISPR dmc1-D1突变在13°C时的交叉发生率下降了95%以上.
- 在30°C时,dmc1-D1突变体呈现出减少的交叉和增加的单价性,尽管不如低温时那么严重.
- 对ttmei1突变的结果类似,但更具变异性,支持DMC1-D1.1的作用.
结论:
- 在小麦中,TaDMC1-D1对于在低温和在一定程度上在高温下保持正常交叉形成至关重要.
- 这些发现对小麦育种有重大影响,特别是对于在气候变化下保持谷物产量.
- 这项研究提供了小麦中第一个CRISPR-Cas9向的DMC1突变,推进了小麦半转变的研究.
关键词:
克里斯普尔是什么意思?克里斯普尔是什么意思?一个DMC1的DMC1在LTP1中使用LTP1.跨界车跨界车跨界车跨界车六倍体小麦是一种六倍体小麦.高温的高温的高温的高温低温低温的低温是一个问题.介质变化 (meiosis) 是一种变质的过程.更多相关视频
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