人类带介质干细胞衍生的外体细胞通过抑制亡来改善自然衰老中的卵巢功能
Zhongkang Li1, Yibin Liu2, Yanpeng Tian2
1Department of Obstetrics and Gynecology, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China.
International journal of molecular medicine
|August 24, 2023
概括
人类带介质干细胞衍生的外体细胞 (hUCMSC-Exos) 可以恢复老年小鼠的卵巢功能. 这些外体提供miR-21-5p来抑制PTEN表达,抑制细胞亡并保持生殖健康.
科学领域:
- 生殖生物学 生殖生物学
- 干细胞生物学 干细胞生物学
- 再生医学是一种再生医学.
背景情况:
- 延长生殖寿命对于妇女的身体和心理健康至关重要.
- 介质干细胞 (MSC) 衍生的外体细胞 (MSC-Exos) 在恢复老化的卵巢功能方面表现有前途.
- 自然卵巢衰老 (NOA) 的小鼠模型被用来研究卵巢衰退.
研究的目的:
- 在NOA.的小鼠模型中评估人类带MSC衍生外体的治疗疗效 (hUCMSC-Exos).
- 研究外体微RNAs (miRNAs) 在hUCMSC-Exo治疗卵巢衰老的机制中的作用.
主要方法:
- 在小鼠中使用hUCMSC-Exos与NOA.
- 评估卵巢功能,包括卵泡数量和激素水平.
- 在体外和体内对PTEN表达和亡的评估.
- 对外体的miRNA测序和生物信息学分析以识别目标miRNA.
主要成果:
- 在NOA小鼠中,hUCMSC-Exos的使用改善了卵巢功能,恢复了卵泡数量和激素水平.
- 外基因组抑制了PTEN表达,并在体内和体外都抑制了亡.
- 鉴定出miR-21-5p是外体细胞中针对PTEN的最高度表达的miRNA.
- 通过提供miR-21-5p,抑制PTEN和亡,hUCMSC-Exos保留了卵巢功能.
结论:
- 在NOA的小鼠模型中,hUCMSC-Exos可以恢复卵巢功能.
- 外体移植作为治疗卵巢衰老的治疗策略具有前景.
- 该机制涉及miR-21-5p的外体递送,以抑制PTEN并减少细胞亡.
关键词:
在这里,我们可以看到PTEN.灭症 (apoptosis) 是一种死亡的过程.外基因组是外基因组中的一个.人类带介质细胞干细胞在 miR-21-5p 中.自然的卵巢衰老是自然发生的.它们的扩散和扩散.更多相关视频
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