SlTCP24和SlTCP29通过与Slas2相互作用并激活西红中SlCKX2的转录来协同调节复合叶的发育
Guoyu Hu1, Danqiu Zhang1, Dan Luo1
1National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, Huazhong Agriculture University, Wuhan, 430070, China.
The New phytologist
|August 24, 2023
概括
两个番茄转录因子,SlTCP24和SlTCP29,协同控制复合叶复杂性. 他们的淘汰会通过影响细胞激素水平而增加叶片数量和复杂性.
科学领域:
- 植物分子生物学 植物分子生物学
- 发展生物学 发展生物学
- 遗传学 遗传学 是一个
背景情况:
- 复合叶的发育是复杂的,涉及叶片的启动和安排.
- 控制这一过程的精确分子机制尚未完全理解.
- 具有TCP图案的转录因子在植物发育中起着至关重要的作用.
研究的目的:
- 调查SlTCP24和SlTCP29在番茄复合物叶子发育中的作用.
- 阐明涉及这些转录因子的分子相互作用和调节途径.
- 在细胞因素代谢中识别由SlTCP24和SlTCP29调节的基因.
主要方法:
- 基因淘汰实验 (同时淘汰SlTCP24和SlTCP29).
- 分析叶子形态和叶子号码.
- 蛋白质与蛋白质相互作用研究 (二聚化试验,与Slas2相互作用).
- 促进体结合试验和基因表达分析 (SlCKX2,细胞因素水平).
主要成果:
- 同时淘汰SlTCP24和SlTCP29导致了叶片数量和叶片复杂性的增加.
- SlTCP24和SlTCP29形成同型和异型二极体,它们由Slas2.2调节.
- 这些转录因子与SlCKX2促进体结合并激活其转录.
- 基因淘汰导致SlCKX2表达减少和细胞因素水平升高.
结论:
- SlTCP24和SlTCP29是番茄复合叶子发育的关键协同调节剂.
- 它们的调节涉及与Slas2的相互作用和通过SlCKX2.2.调节细胞因素代谢途径.
- 提出了一种涉及这些因素和细胞因素的复合叶子发育的监管模型.
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