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通过LEDGFF进行核细胞识别的多价值性

Eliška Koutná^1,2, Vanda Lux¹, Tomáš Kouba¹

¹Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Prague 160 00, Czech Republic.

Nucleic acids research

|August 24, 2023

概括

LEDGF PWWP 域识别了二甲基和三甲基化 H3K36 基因素标记. 在LEDGF的内在无序区域与DNA相互作用,可能影响转录调节.

科学领域:

分子生物学分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
结构生物学结构生物学

背景情况:

细胞的转录依赖于由染色体阅读器识别的基因组修饰.
不同基因组修饰状态之间的歧视机制尚未完全理解.
内在无序区域在核细胞结合中的作用在很大程度上是未被探索的.

研究的目的:

阐明LEDGF PWWP域对H3K36甲基化识别的结构基础.
研究LEDGF内在无序区域在核细胞和DNA相互作用中的作用.
了解LEDGF如何促进转录延长.

主要方法:

进行X射线晶体学以确定LEDGF PWWP域与H3K36二甲基和三甲基核体复合的结构.
生物化学试验用于研究LEDGF内在无序区域的DNA结合特性.
在二核子组背景下分析动态相互作用.

主要成果:

在LEDGF PWWP域识别H3K36二甲基和三甲基化类似.
在核体DNA接口上确定了PWWP域的二次相互作用点.
在LEDGF中,本质上有障碍的区域含有DNA相互作用的基因,可以区分内核和外核体DNA.

结论:

LEDGF利用其PWWP域用于H3K36甲基化识别和DNA相互作用的额外网站.
本质上无序的区域有助于核细胞结合和DNA歧视.
LEDGF与染色质的多价值相互作用可能通过招募因子来促进转录延长RNA聚合酶II.

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