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在Cas9变种中,指南特定的效率损失和非目标减量

Liang Zhang1, Wei He1, Rongjie Fu1

  • 1Department of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.

Nucleic acids research
|August 24, 2023
PubMed
概括

高保真性Cas9变体 (HiFi,LZ3) 减少了CRISPR的目标外效应,但可以降低目标上的效率. GuideVar软件预测了这些变体的效率和非目标活动,帮助sgRNA选择.

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