相关实验视频
Updated: Jul 18, 2025
06:59
Nanomanipulation of Single RNA Molecules by Optical Tweezers
Published on: August 20, 2014
14.9K
可光切换的RNA分子:可逆光控制的结构和功能的 ribozyme 的作用
Chikara Dohno1, Maki Kimura1, Yusuke Fujiwara1
1Department of Regulatory Bioorganic Chemistry, SANKEN (The Institute of Scientific and Industrial Research), Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan.
Nucleic acids research
|August 24, 2023
概括
研究人员开发了一种可光开关的分子 (NCTA),可以通过光控制RNA结构和功能. 这种光激活的分子精确地调节细胞中的RNA折叠和头核糖酶活性.
科学领域:
- 分子生物学分子生物学
- 生物化学 生化学
- 在RNA治疗方面,RNA疗法.
背景情况:
- RNA分子采用各种结构,这些结构对于它们的生物学作用至关重要.
- 动态控制RNA构造是理解和操纵RNA功能的关键.
- 现有的RNA结构控制方法在可逆性和精度上是有限的.
研究的目的:
- 开发一种新型的可光切换分子,用于可逆控制RNA结构和功能.
- 为了研究NCTA分子对点RNA的光依赖结合和活性.
- 为了证明RNA折叠和酶活性在细胞环境中的精确光控制.
主要方法:
- 设计和合成NCTA,一种RNA结合配体,具有可光异构的亚博基部分.
- 描述Z-NCTA和E-NCTA异构体对目标RNA序列的结合亲和力和特异性.
- 评估NCTA使用光化学控制调节RNA二级和三级结构的能力.
- 在接受NCTA治疗并接受光辐射的细胞中,对头核糖酶活性可逆调节的评估.
主要成果:
- NCTA充当可光切换的分子,在光刺激后可逆控制RNA结构.
- Z-NCTA异构体特别结合并稳定了基RNA的杂交状态,而E-NCTA异构体则没有.
- 对NCTA的光可逆异构化允许对RNA的二级和三级结构进行动态控制.
- 头核糖酶的RNA分裂活性在细胞实验中被NCTA和光成功和可逆地调节.
结论:
- NCTA提供了一种强大的工具,可以精确地对RNA结构和功能进行光感应控制.
- 这种可光切换的分子能够动态调节基于RNA的生物过程,包括酶活性.
- 这些发现表明,生物系统中RNA操纵的光控制策略取得了重大进展.
更多相关视频
10:10Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags
Published on: January 17, 2019
10.5K
10:59Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy
Published on: October 18, 2024
692
相关概念视频
01:56Riboswitches
8.2K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.2K
01:29Translational Regulation
44
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
44
01:23Transcriptional Regulation: Riboswitches
61
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
61
01:23Types of RNA
64.0K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
64.0K
02:47Ribozymes
12.3K
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can...
Ribozymes can...
12.3K
02:15Experimental RNAi
6.2K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.2K