通过封装和捕获来固定Rhodococcus:一种对苦副产品的绿色解决方案
María C Pilar-Izquierdo1, María López-Fouz2, Natividad Ortega2
1Department of Biotechnology and Food Science, University of Burgos, Plaza Misael Bañuelos, S/N, 09001, Burgos, Spain. mcpilar@ubu.es.
Applied microbiology and biotechnology
|August 24, 2023
概括
使用固定Rhodococcus fascians细胞对类副产品进行微生物借记,为化学治疗提供了一个有希望的替代方案. 在聚乙醇/聚乙烯糖醇冷凝中捕获,对于胺的降解和操作稳定性来说是优越的.
科学领域:
- 生物技术是生物技术.
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
背景情况:
- 类的副产品含有有价值的化合物,但需要提取,以去除像林因这样的苦.
- 化学借记方法可能很苛刻,可能会影响附加值化合物的质量.
- 微生物解决方案提供了一种可能更温和,更可持续的类借记方法.
研究的目的:
- 为了研究Rhodococcus fascians细胞的固定,用于类副产品的负债处理.
- 为了比较两种固定化技术:Ca-酸盐空心珠封装和聚乙醇/聚乙烯甘醇 (PVA/PEG) 冰凝捕获.
- 优化固定条件并评估微生物借记的效率和稳定性.
主要方法:
- 用子皮提取物适应Rhodococcus fascians菌株的limonoids.
- 对Ca-酸盐空心珠 (Na-酸盐,CaCl2,CMC,微生物负载) 和PVA/PEG冷凝 (PVA,PEG,微生物负载) 细胞固定条件的优化.
- 在最佳固定条件下,在合成果汁和汁中评估胺降解,包括通过多次重复使用周期进行操作稳定性测试.
主要成果:
- 两种方法都确定了最佳的固定条件,其中包括特定度的酸盐,CaCl2,CMC,PVA,PEG和微生物负载 (0.6 OD600).
- 与空心珠封装相比,在PVA/PEG冷凝中捕获提供了更好的保护微生物细胞免受酸性中介应激.
- 在合成果汁 (pH3) 中72小时后,PVA/PEG冷凝固定细胞的利蒙宁降解率达到32%,空心珠封装细胞的降解率达到28%.
- 用冷凝固定的细胞表现出更高的运行稳定性,在汁中成功完成了四个连续的重复使用周期.
结论:
- 适应类副产品的Rhodococcus fascians可以有效地降解胺.
- 通过在PVA/PEG冷凝中捕获固定是类产品微生物借记的优质方法,在酸性pH下提供更好的保护和效率.
- 用冷凝固定的Rhodococcus fascians是一种稳定有效的生物催化剂,用于类副产品的消耗,促进增值化合物的回收.
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