细胞内细菌根除使用新型体在体外.
Wing Nok Isaac Ng1, Shanthini Kalimuthu1, Carmen Oi Kwan Law2
1Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, Hong Kong.
International endodontic journal
|August 24, 2023
概括
新型抗微生物OP145与细胞透R8结合,有效地根除了细胞内Enterococcus faecalis. 与单独的OP145和标准抗生素相比,这种结合物显示出优异的抗菌活性,没有宿主细胞毒性.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 细胞生物学 细胞生物学
背景情况:
- Enterococcus faecalis是内牙感染中常见的病原体.
- 细胞内细菌在治疗持久性感染方面构成了重大挑战.
- 需要新的抗微生物策略来克服抗生素耐药性并改善治疗结果.
研究的目的:
- 评估一种新型抗微生物 (AMP) OP145与细胞透 (Octa-arginine/R8) 相结合的新型抗微生物 (OP145) 对细胞内Enterococcus faecalis.alis.的疗效.
- 为了比较OP145-R8结合剂与单独的OP145和再生性内牙科中使用的标准抗生素组合物的抗菌活性.
- 评估OP145-R8结合物在宿主细胞中的安全性和内部化特性.
主要方法:
- 使用人类巨细胞和红细胞评估了OP145和OP145-R8的生物相容性.
- 用对焦激光扫描显微镜和流细胞计量来量化体内化成巨细胞.
- 通过和抗生素对E. faecalis的细胞外和细胞内杀死是通过殖民地形成单位 (CFU) 数量来确定的.
主要成果:
- 与单独的OP145相比,OP145-R8结合体显示了增强的和依赖时间的内部化到巨细胞.
- 与OP145相比,OP145-R8表现出明显更大的细胞外和细胞内抗菌活性对E. faecalis (p <0.05).
- 在7.5μmol/L,OP145-R8在2小时内根除了细胞内E. faecalis (3.5日志减少),超过了抗生素 (p <0.05).
结论:
- 将AMP OP145与细胞透R8结合,显著提高了其对细胞内E. faecalis的有效性.
- OP145-R8表现出强大的抗菌活性和有效的内部化到宿主细胞,没有明显的毒性.
- 这种新型联体代表了治疗持续性E. faecalis感染的有希望的治疗候选者,特别是在内牙再生程序中.
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