openFrame:一个模块化,可持续的开放式显微镜平台,具有单射,双轴光学自动对焦模块,提供高精度和长距离操作
J Lightley1,2, S Kumar1,2, M Q Lim1,3
1Photonics Group, Physics Department, Imperial College London, London, UK.
Journal of microscopy
|August 24, 2023
概括
本研究介绍了openFrame,一个模块化,低成本,开放硬件显微镜平台. 它能够实现可适应,可升级的研究级仪器,并具有用于精确,自动成像的新型自动对焦,包括单分子定位显微镜.
科学领域:
- 显微镜的使用方法
- 光学工程是指光学工程.
- 生物技术是生物技术.
背景情况:
- 传统的显微镜系统可能很昂贵,缺乏模块化.
- 适应显微镜的先进技术,如单分子局部化显微镜 (SMLM) 通常需要专门的,昂贵的设备.
研究的目的:
- 开发一个多功能,低成本,开放硬件显微镜平台 (openFrame).
- 为精确,自动化显微镜设计一个强大的光学自动对焦系统.
- 为了证明平台对SMLM等先进成像技术的实用性.
主要方法:
- 设计了一个模块化,开放的硬件显微镜平台,具有可互换组件.
- 开发了一种新的光学自动对焦,使用直角圆柱形光学来锁定焦点.
- 在openFrame平台上使用多模二极管激光和冷却的CMOS摄像头实现了自动化的SMLM.
主要成果:
- 开放框架平台允许以低成本组装和维护研究级仪器.
- 这种新型自动对焦系统实现了<200nm的精度,失焦率高达±37μm (单拍) 和±68μm (两步).
- 自动化SMLM在经济高效的openFrame系统上成功实现.
结论:
- openFrame提供了一种灵活且负担得起的解决方案,用于构建和升级各种显微镜系统.
- 开发的自动对焦技术显著提高了自动化显微镜的稳定性和准确性.
- 该平台以更容易获取的方式为SMLM等先进研究应用程序提供便利.
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