在哺乳期的荷尔斯坦奶牛中,在肠道屏障挑战期间,以Saccharomyces cerevisiae发酵产品养的ileal转录组的变化,确定潜在的调节过程
Qianming Jiang1, Valentino Palombo2, Danielle N Sherlock1
1Department of Animal Sciences, University of Illinois, Urbana 61801, IL, USA.
Journal of animal science
|August 24, 2023
概括
一种Saccharomyces cerevisiae发酵产品 (SCFP) 通过在料限制期间降低免疫,细胞外基因和粘素合成基因的调节,减少了奶牛的漏肠. 这种反应的关键是Homeobox转录因子.
科学领域:
- 动物科学动物科学
- 营养生物化学 营养生物化学
- 分子生物学分子生物学
背景情况:
- 奶牛的压力因素可能会损害肠道屏障功能,导致肠道疾病.
研究的目的:
- 调查Saccharomyces cerevisiae发酵产品 (SCFP) 在料限制 (FR) 期间影响阴茎转录组的分子机制.
主要方法:
- 在9周的时间里,奶牛要么接受控制饮食,要么接受SCFP补充的饮食.
- 一个5天的食限制 (40%的随意摄入量) 的挑战被强加.
- 用RNA测序 (RNAseq) 提取和分析了状RNA.
- 生物信息分析利用KEGG和GO数据库来识别差异表达基因 (DEG) 和途径.
主要成果:
- 与对照组相比,SCFP补充导致1696个差异表达基因 (DEG).
- 关键下调的途径包括"Mucin类型O-甘氨酸生物合成"和与细胞外矩阵结构 (原体,拉米因,整基因) 相关的基因.
- 化学基因信号通路的下调,而"谷氨代谢"的上调.
- 在最高预测的转录因子中,确定了9个homeobox转录因子.
结论:
- 在料限制期间,SCFP降低了乳牛乳皮中的免疫,细胞外基因和粘素合成相关基因的下调.
- 在压力期间,Homeobox转录因子在SCFP的转录反应中发挥着重要作用.
- 在压力下的奶牛中,SCFP显示出减轻肠道屏障功能障碍的潜力.
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