在Aconitum植物中二烯合成酶的功能多样性
Mei Tian1, Baolong Jin1, Lingli Chen2
1State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
Plant physiology and biochemistry : PPB
|August 24, 2023
概括
这项研究揭示了Aconitum物种中多样化的迪特尔合成,扩大了关于迪特尔类类生物合成及其进化多样化的知识. 这些发现揭示了产生药用和有毒化合物的复杂途径.
科学领域:
- 植物生物化学 植物生物化学
- 分子生物学分子生物学
- 植物化学 植物化学
背景情况:
- 阿科尼 (Ranunculaceae家族) 种类产生各种药用和有毒的二甲类化物 (DA).
- 在历史上,恩特-二酸 (ent-CPP) 被认为是DA的唯一前体.
- 最近的发现表明了A. gymnandrum中的ent-8,13-CPP合成酶等替代前体,促使对基因保存进行研究.
研究的目的:
- 在四种额外的Aconitum物种中识别和功能性表征二烯合成酶 (diTPSs).
- 为了探索 diTPS 基因在 Aconitum 属的保护和多样性.
- 为了阐明二烯生物合成途径,负责Aconitum中多样化的DA配置.
主要方法:
- 用RNA测序和PacBio异序测序来识别四种Aconitum物种中的diTPS.
- 进行了体外和体内测试,以描述已识别的diTPSs的酶活性.
- 进行了遗传学分析,以将diTPS进化与物种的遗传学相关联.
主要成果:
- 确定了10个II类和9个I类的diTPS,并对其功能进行了描述.
- 七种II类二TPS被证实是ent-CPP合成酶;另外三种产生ent-8,13-CPP,8,13-CPP和8α--CPP.
- 特定的I类甲合成酶类 (KSLs) 显示出不同的基质特异性,从不同的前体中产生甲,乙和Z-abienol.
结论:
- 在六种Aconitum物种中划出已知的二烯生物合成途径,揭示了diTPSs的显著分歧.
- 鉴定到的 diTPS 多样性与 Aconitum 基因相一致,并且可能推动了 DA 的差异分布.
- 建立了未来研究的基础,即在Aconitum属内对DA生物合成的多样化.
相关概念视频
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