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多维代谢概况显示了与严重急性胰腺炎现型相关的失调的鸟类代谢特征

Jinxi Yang¹, Na Shi¹, Shisheng Wang²

¹West China Centre of Excellence for Pancreatitis, Institute of Integrated Traditional Chinese and Western Medicine, Sichuan Provincial Pancreatitis Centre and West China-Liverpool Biomedical Research Centre, West China Hospital/West China Medical School, Sichuan University, Chengdu, China.

Translational research : the journal of laboratory and clinical medicine

|August 24, 2023

概括

代谢分析显示,在患有严重急性胰腺炎 (SAP) 的患者中,有明显的代谢特征. 甲素代谢途径的失调与SAP进展有关,为早期诊断提供了潜在的可能性.

科学领域:

代谢学和临床生物化学
胃肠病学和肝病学胃肠学和肝病学
发现生物标志物的发现.

背景情况:

严重急性胰腺炎 (SAP) 是一种严重的疾病,具有显著的发病率和死亡率.
确定可靠的生物标志物用于早期SAP诊断,并了解其病原性仍然是一个挑战.
代谢失调越来越被认为是各种疾病的标志,包括胰腺炎.

研究的目的:

识别与严重急性胰腺炎 (SAP) 相关的代谢特征.
探索甲素代谢途径在SAP病变发生过程中的作用.
开发一种以代谢学为基础的模型,用于早期SAP预测.

主要方法:

在106名急性胰腺炎 (AP) 患者和41名对照患者的血样本上,基于LC-MS/MS的向代谢量.
在入院后的1,3,7天进行时间代谢分析.
随机森林分析,质谱成像 (MSI),免疫组织化学和L-ornithine诱导的AP动物模型.

主要成果:

一个由22个不同代谢物的小组准确地预测了SAP与非SAP (NSAP) 的SAP,AUC为0.955.
六个关键代谢物显示胰腺病变的显著变化,确定了减少的素相关代谢物.
在SAP中观察到激活的ornithine代谢途径和降低的catabolites,以及改变的酶表达 (arginase2,ornithine transcarboxylase).

结论:

AP患者的血代谢组是独特的,并为早期SAP诊断提供了希望.
AP恶化与激活的鸟代谢途径和不足的催化剂水平有关.
代谢分析提供了对SAP病原体和潜在治疗点的见解.

关键词:

阿尔金纳2的使用质谱仪成像成像质谱仪成像甲素的新陈代谢严重的急性胰腺炎有针对性的代谢组学.

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