从Bacillus velezensis PhCL发酵中优化和净化生物产品及其在工业应用和生物修复方面的潜力
Chonlong Chio1, Sarita Shrestha1, Griffin Carr1
1Department of Biology, Lakehead University, 955 Oliver Road, Thunder Bay, Ontario P7B 5E1, Canada.
The Science of the total environment
|August 24, 2023
概括
这项研究引入了一种使用硫酸沉净化酶和生物表面活性剂的新方法. 这种具有成本效益的方法产生了更多的生物表面活性剂,并为生物修复应用提供了潜力.
科学领域:
- 生物技术是生物技术.
- 生物化学 生化学
- 环境科学 环境科学
背景情况:
- 生物生产为绿色化学品提供了一条可持续的途径,但高的下游加工成本阻碍了可扩展性.
- 目前的生物表面活性剂净化方法,如酸性沉,涉及强化学品和限制应用.
- 开发具有成本效益和高效的净化策略对于推动生物产品利用至关重要.
研究的目的:
- 为了隔离和优化一种新型的Bacillus velezensis PhCL菌株用于氨基酶生产.
- 开发和评估一种序列性硫酸盐沉方法,用于共同净化粉酶和生物表面活性剂.
- 描述纯化的氨酶和生物表面活性剂,并评估生物表面活性剂在生物修复中的有效性.
主要方法:
- 从废物中分离出Bacillus velezensis PhCL. 这是一个非常好的方法.
- 通过响应表面方法来优化氨酶的产生.
- 序列硫酸盐沉用于同时净化粉酶和生物表面活性剂.
- 净化化合物的特性和生物表面活性剂在去除碳化合物的性能评估.
主要成果:
- 通过新型Bacillus velezensis PhCL菌株优化氨酶的产生.
- 通过硫酸沉成功地联合净化了酶和生物表面活性剂,与酸沉相比,获得了更高的生物表面活性剂产量.
- 纯化原始生物表面活性剂显示出增强的乳液活性和更高的石油碳化合物去除率.
- 酶和生物表面活性剂都被回收,从而降低了整体净化成本.
结论:
- 序列性硫酸盐沉提供了一种经济有效的替代方案,用于从发酵中净化酶和生物表面活性剂.
- 同净化的生物表面活性剂显示出石油和多环芳的生物修复的巨大潜力.
- 这项研究提出了一种可行的方法,可以同时恢复有价值的生物分子,从而提高生物生产的经济可行性.
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