同时固定增强协同相互作用和细菌联盟的原油去除
Adisan Rungsihiranrut1, Chanokporn Muangchinda1, Kallayanee Naloka2
1International Postgraduate Programs in Hazardous Substance and Environmental Management, Graduate School, Chulalongkorn University, Bangkok, 10330, Thailand; Center of Excellence in Microbial Technology for Marine Pollution Treatment (MiTMaPT), Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand.
Chemosphere
|August 24, 2023
概括
不动化的细菌联盟有效地从海水中去除了原油. 同时固定Sphingobium naphthae MO2-4和Priestia aryabhattai TL01-2提高了生物降解效率,为海洋石油泄漏整治提供了可持续的解决方案.
科学领域:
- 环境微生物学 环境微生物学
- 生物修复是一种生物修复.
- 海洋生物技术 海洋生物技术
背景情况:
- 海洋石油泄漏对环境构成重大威胁.
- 微生物降解提供了一种可持续的石油泄漏清理方法.
- 细菌联盟可以提高原油生物降解效率.
研究的目的:
- 为了评估从海水中去除原油的固定细菌联盟的效率.
- 调查固定化方法和注射剂比对生物降解的影响.
- 分析细菌菌株碳化合物降解能力的基因组基础.
主要方法:
- 通过生物膜形成在水孔凝上固定Sphingobium naphthae MO2-4和Priestia aryabhattai TL01-2.
- 测试同步与独立固定和不同的注射器体积比率 (2:1 MO2-4:TL01-2).
- 评估批量 (2000毫克L-1),半连续和波 (1000毫克L-1) 试验中的原油提取.
- 细菌菌株的基因组分析,以确定与碳化合物降解和耐药性相关的基因.
主要成果:
- 同时对细菌联盟的固定显示出比单个菌株更高的原油去除效率.
- 固定化的财团在7天内去除了77%的原油 (2000毫克L-1),并在半连续测试中保持了有效性.
- 在一个20升的波浪水箱中,固定化的联盟在28天内实现了大约70%的原油降解,没有观察到自然衰减.
- 基因组分析证实了两种菌株中碳化合物降解,重金属耐药性,生物表面活性剂合成和生物膜形成的基因.
结论:
- 细菌联合体的同时固定增强了海洋环境中的原油生物降解.
- 固定化的财团证明了石油污染海水大规模整治的巨大潜力.
- 鉴定的基因支持观察到的细菌菌株的生物降解能力和耐药性特征.
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