来自不为人知的泡Takifugu obscurus的三种新型L型讲蛋白促进了抗菌性免疫反应
Ying Huang1, Xin-Yue Yu1, Peng Luo2
1Jiangsu Province Engineering Research Center for Marine Bio-resources Sustainable Utilization, Nanjing, 210098, China; Department of Marine Biology, College of Oceanography, Hohai University, Nanjing, 210098, China.
Developmental and comparative immunology
|August 24, 2023
概括
从气球鱼中鉴定出了三种L型讲蛋白 (LTL),发现它们与细菌结合. 这些LTLs作为模式识别受体,增强鱼类的抗菌免疫力.
科学领域:
- 免疫学 免疫学 免疫学
- 海洋生物学 海洋生物学
- 分子生物学分子生物学
背景情况:
- L型甲状腺素 (LTLs) 拥有豆类甲状腺素域,对于结合高曼诺斯寡糖至关重要.
- LTLs在糖蛋白分泌途径和各种免疫反应中发挥着重要作用.
研究的目的:
- 克隆和表征来自不为人知的气球鱼Takifugu obscurus的三种L型莱克同类物 (ToVIP36-1,ToVIP36-2,ToVIP36-3).
- 在细菌感染的背景下研究这些LTLs的表达模式和功能作用.
主要方法:
- 克隆和对ToVIP36基因的序列分析.
- 定量实时PCR用于不同组织和细菌挑战后的表达分析.
- 再组合LTL域 (rToVIP36-LTLDs) 的 Prokaryotic 表达和净化.
- 在体外测试以评估细菌凝结和生长抑制.
主要成果:
- 鉴定了三个ToVIP36基因,它们编码具有保存的LTL域,跨膜区域和KRFY贩运信号的蛋白质.
- ToVIP36s表现出广泛的组织表达,肝脏和肠道的水平更高,并且在对Vibrio harveyi和黄金葡萄球菌的反应中表达变化.
- 再组合的ToVIP36-LTLD蛋白显示了几种鱼类致病细菌的依赖的结合.
- 这些蛋白质通过结合 lipopolysaccharides 和 peptidoglycans 等细菌成分来抑制细菌的生长.
结论:
- 鉴定到的ToVIP36蛋白质作为Takifugu obscurus中的模式识别受体起作用.
- 这些发现凸显了L型莱克在鱼类抗菌免疫力中的关键作用.
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