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利用来自人类诱导的多能干细胞的牙细胞来进行硬组织工程

Eun-Jung Kim¹, Ka-Hwa Kim¹, Hyun-Yi Kim²

¹Division in Anatomy and Developmental Biology, Department of Oral Biology, Taste Research Center, Oral Science Research Center, BK21 FOUR Project, Yonsei University College of Dentistry, Seoul 03722, Korea.

Journal of advanced research

|August 24, 2023

概括

人类诱导的多能干细胞 (hiPSCs) 可以分化为牙细胞,用于硬组织再生. 这些细胞与生物材料相结合,可以形成矿物化组织,甚至生物工程牙.

科学领域:

干细胞生物学干细胞生物学
再生医学是一种再生医学.
生物材料科学是生物材料的科学.

背景情况:

像骨和牙这样的矿化组织对人类健康至关重要.
人类诱导的多能干细胞 (hiPSCs) 具有再生骨和牙缺陷的潜力.
了解细胞外矩阵 (ECM) 微环境对于干细胞疗法至关重要.

研究的目的:

优化一种与人类兼容的协议,用于将hiPSC分化为没有动物产品的牙细胞.
为了实现hiPSC衍生牙细胞的矿化,将其转化为硬组织.
探索hiPSC衍生牙细胞在骨和牙再生疗法的潜力.

主要方法:

通过胚胎体和神经细胞,hiPSCs通过胚胎体和神经细胞分化为牙上皮细胞 (DEC) 和牙介质细胞 (DMC).
差异化协议涉及定制媒体,包括BMP调制的媒体用于DECs.
由hiPSC衍生的牙细胞使用转录组分析,西部涂抹和RT-qPCR进行了表征.
矿化是通过将DEC和DMC重新组合在GelMA,原蛋白和 agar等生物材料中引起的.

主要成果:

从hiPSC衍生的牙细胞在GelMA水凝和I型原蛋白中表现出高的骨质生成和胆固醇-骨质生成潜力.
在阿加凝基质中封装促进了生物工程牙的形成.
该研究成功地从hiPSCs中产生了矿化牙组织.

结论:

本研究提出了一种利用hiPSCs在硬组织再生中的方法,适用于牙和骨缺陷.
这些发现突显出一种具有重要的生物工程器官再生医学生物工程器官潜力的新技术.
开发的协议为牙细胞分化提供了一种无料和无动物成分的方法.

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