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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions

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下一代蛋白质组学用于定量化Jumbophage-细菌相互作用映射.

Andrea Fossati^1,2,3, Deepto Mozumdar⁴, Claire Kokontis⁴

¹J. David Gladstone Institutes, San Francisco, 94158, CA, USA.

Nature communications

|August 24, 2023

概括

同分离质谱学揭示了 Pseudomonas aeruginosa jumbophages 的 6000 多种宿主-病原体相互作用. 这种高通量方法揭示了保存的菌体掠食机制,并确定了新的蛋白质复合体.

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Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

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科学领域:

微生物学微生物学
病毒学病毒学
蛋白质组学是指蛋白质组学.

背景情况:

宿主-病原体相互作用对于感染动态至关重要.
传统的亲和净化质谱在研究这些相互作用的可扩展性和生物真实性方面存在局限性.
菌体,就像菌体一样,为剖析宿主-病原体相互作用提供了独特的模型.

研究的目的:

应用同分离质谱法用于高通量分析本地巨菌感染期间宿主-病原体相互作用.
描述 Pseudomonas aeruginosa 和 jumbophages φKZ 和 φPA3.3 之间广泛的蛋白质-蛋白质相互作用网络.
开发一个可访问的资源,用于探索菌体-宿主互动组数据.

主要方法:

利用同分离质谱分析了感染食菌 φKZ 和 φPA3.3 的 Pseudomonas aeruginosa 宿主-病原体相互作用.
量化和识别了每种菌体6000多种独特的宿主-病原体相互作用,覆盖了其50%以上的蛋白质组.
开发了PhageMAP,这是一个在线数据库,用于查询,可视化和预测菌体与宿主相互作用.

主要成果:

检测到广泛的宿主-病原体相互作用,包括与宿主核糖体相互作用的菌体蛋白.
确定了以前未经表征的菌体开放阅读框架 (ORF) 的新型蛋白质复合体.
揭示了KZ类菌体之间保存的相互作用模式,表明共享的掠食机制.
发现了一个φPA3复合体,其结构和序列类似于φKZ非病毒型RNA聚合酶.

结论:

同分离质谱是一种可扩展和有效的方法,用于在病毒感染期间深入分析宿主-病原体相互作用体.
这项研究提供了巨细胞的综合互动组图,揭示了保存的机制和新型蛋白质功能.
PhageMAP 作为研究界的宝贵资源,促进了对菌体生物学和宿主操纵的进一步调查.