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基于LC-MS/MS的向代谢产物中含有碳酸的代谢产物的异化衍生策略

Aimin Wang^1,2, Yuting Fan^1,2, Jun Fu³

¹State Key Laboratory of Electroanalytical Chemistry, National Center of Mass Spectrometry in Changchun, Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China.

Analytical and bioanalytical chemistry

|August 24, 2023

概括

这项研究引入了一种使用异化衍生物和LC-MS/MS的新方法,用于在阿尔茨海默病研究中同时量化39种含有碳素的代谢物 (CCM). 该技术提高了检测灵敏度,并揭示了传统草药如何影响AD大鼠中的CCM.

科学领域:

生物化学生化学
分析化学分析化学
神经科学是一个神经科学.

背景情况:

代谢学识别疾病表型,并告知有针对性的疗法.
含有碳酸的代谢物 (CCM) 是至关重要的,但由于其多样性的特性,同时量化是具有挑战性的.
传统的LC-MS方法难以同时分析多个CCM类型.

研究的目的:

开发一种新的有针对性的代谢学策略,用于同时量化各种CCM.
改善与阿尔茨海默病 (AD) 相关的CCM的检测覆盖率和灵敏度.
为了阐明SG草本对对AD大鼠中CCM代谢障碍的调节作用.

主要方法:

提出了使用异化衍生物结合LC-MS/MS的有针对性的代谢学策略.
同时量化了来自5种不同类型的39个CCM,具有显著的极性差异.
在AD大鼠的便,尿液,血清和大脑中分析了CCM,以及药理动力学和肠道微生物群分析.

主要成果:

异化衍生物方法实现了高产量,并显著改善了CCM检测极限 (1.25-2000倍).
在CCM量化中表现出卓越的选择性,准确性,精度和可重复性.
阐明了SG草本对对AD大鼠中CCM代谢障碍的调节作用,提供了对其机制的全面理解.

结论:

新的异化衍生物结合向代谢学是有效的同时CCM量化.
这种方法显著提高了微量生物血统分析的检测能力,特别是在AD研究中.
通过代谢分析,提供了对AD传统草药治疗机制的新见解.

关键词:

阿尔茨海默氏症是阿尔茨海默氏症的一种疾病.含有碳酸的代谢产物异化的衍生物化有针对性的代谢组学.

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