相关实验视频

Updated: Jul 18, 2025

Wholemount In Situ Hybridization for Astyanax Embryos

Wholemount In Situ Hybridization for Astyanax Embryos

Published on: March 2, 2019

对Acrossocheilus jishouensis (Teleostei:Cyprinidae) 的分子鉴定及其完整的线粒体基因组

Xiangying Lan^1,2, Jinxiu Wang^1,2, Mingyao Zhang^1,2

¹Hunan Engineering Laboratory for Chinese Giant Salamander's Resource Protection and Comprehensive Utilization, and Key Laboratory of Hunan Forest Products and Chemical Industry Engineering, National and Local United Engineering Laboratory of Integrative Utilization Technology of Eucommia Ulmoides, Jishou University, Zhangjiajie, China.

Biochemical genetics

|August 24, 2023

概括

分子鉴定扩大了Acrossocheilus jishouensis在五个新的地区的已知分布. DNA条形码证实了这些种群是A. jishouensis,揭示了遗传相似性并帮助了cyprinid进化研究.

科学领域:

鱼类学鱼类学鱼类学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

分子鉴定,包括DNA条形码,对于物种差异化至关重要.
此前,Acrossocheilus jishouensis被认为仅限于一个地方.

研究的目的:

在五个地区进行Acrossocheilus jishouensis的分子鉴定.
扩大已知A. jishouensis的分布.
为cyprinid进化研究提供基因组数据.

主要方法:

使用线粒体COI基因序列进行分子鉴定.
整个线粒体基因组组合 (16,594个pb).
使用贝叶斯推理 (BI) 和最大概率 (ML) 方法进行的家族遗传分析.

主要成果:

来自五个种群的形态相似样本被确定为A. jishouensis,遗传距离很小 (0.1-1.6%).
该物种的分布大幅扩大.
线粒体基因组分析揭示了基因组成,启动/停止编码子和基组成模式.
遗传学分析表明,Acrossocheilus和Onychostoma属是非单一的.

结论:

这项研究大大扩大了已知的Acrossocheilus jishouensis的分布.
在Acrossocheilus和Onychostoma之间进行传统诊断需要进一步评估.
基因组数据作为未来的cyprinid分子和进化研究的宝贵资源.

关键词:

它们中的一个是Acrossocheilus jishouensis.DNA 条形码编码地理分布地理分布.线粒体基因组的基因组分子识别分子识别遗传学方面的影响.

更多相关视频

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

Published on: February 10, 2023

Novel Sequence Discovery by Subtractive Genomics

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

相关概念视频

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...

Export of Mitochondrial and Chloroplast Genes

Export of Mitochondrial and Chloroplast Genes

A eukaryotic cell can have up to three different types of genetic systems: nuclear, mitochondrial, and chloroplast. During evolution, organelles have exported many genes to the nucleus; this transfer is still ongoing in some plant species. Approximately 18% of the Arabidopsis thaliana nuclear genome is thought to be derived from the chloroplast’s cyanobacterial ancestor, and around 75% of the yeast genome derived from the mitochondria’s bacterial ancestor. This export has occurred...

Animal Mitochondrial Genetics

Animal Mitochondrial Genetics

Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

Multi-species Conserved Sequences

Multi-species Conserved Sequences

Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...

Evolutionary Relationships through Genome Comparisons

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...