希波克萨米R-210-3p通过P53和Akt调节介酶干细胞的增殖
Sanjay Kumar1,2,3, Varsha Yadav4, Namrta Sharma4
1Department of Biochemistry, Medical College, All India Institute of Medical Sciences, Bathinda, India. kumar.sanjay11@aiimsbathinda.edu.in.
Molecular and cellular biochemistry
|August 24, 2023
概括
介质干细胞 (MSC) 增殖在低氧和正常的条件下由microRNA-210-3p调节. 这种微RNA影响细胞循环调节器,如p53,p21和pAkt,对于干细胞的生存和修复至关重要.
科学领域:
- 干细胞生物学 干细胞生物学
- 分子生物学分子生物学
- 心血管研究研究心血管研究
背景情况:
- 由于不利的条件,缺血性心肌中移植干细胞的高死亡率.
- 对低氧在非转化介质干细胞 (MSC) 细胞周期调节中的作用的理解有限.
- 需要阐明MSC扩散机制,以获得有效的再生疗法.
研究的目的:
- 研究骨髓衍生MSCs (BM-MSCs) 中细胞循环调节的机制.
- 分析低氧诱导的微RNA-210-3p (miR-210-3p) 在MSC增殖中的作用.
- 确定关键的细胞循环调节者,参与MSC在不同氧气条件下的生存和繁殖.
主要方法:
- 在低氧 (1% O2) 和正常的条件下,对大鼠BM-MSCs的隔离和体外传播.
- 细胞增殖的评估使用CFSE测定和流细胞计.
- 通过敲击和抗miR转染来操纵miR-210-3p水平.
- 使用西欧斑点进行细胞周期调节器表达 (p53,p21,pAkt) 的分析.
主要成果:
- 低氧显著增加了BM-MSC的扩散相比normoxia.
- miR-210-3p knockdown 降低了缺氧下的 BM-MSC 扩散,而它的上调则增强了扩散.
- miR-210-3p通过调节p53,p21和pAkt在低氧和正常条件下的表达来调节BM-MSC的增殖.
结论:
- miR-210-3p在调节BM-MSC扩散方面发挥着至关重要的作用,无论氧气是否可用.
- 该机制涉及关键细胞循环调节者的调节:p53,p21和pAkt.
- 这些发现突出了miR-210-3p作为增强基于干细胞的再生策略的潜在治疗点.
更多相关视频
07:15Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway
Published on: August 23, 2024
472
11:53An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C
Published on: June 1, 2018
6.7K
相关概念视频
01:19Mesenchymal Stem Cells
4.8K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
4.8K
01:22PI3K/mTOR/AKT Signaling Pathway
3.7K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.7K
01:01Regulation of Hematopoietic Stem Cells
3.2K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.2K
02:23Abnormal Proliferation
4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
01:24Regulation of Angiogenesis and Blood Supply
2.6K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.6K