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KRT18调节热囊细胞迁移和入侵,这对于胚胎植入至关重要

Xiaoling Liang^1,2, Xiaoxiao Qiu^1,3, Yana Ma¹

¹Assisted Reproduction Unit, Department of Obstetrics and Gynaecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Reproductive biology and endocrinology : RB&E

|August 24, 2023

概括

氨酸18 (KRT18) 对于胚胎植入至关重要. 这项研究表明,KRT18直接与E-cadherin结合,促进热囊细胞的侵入和粘附,这是成功怀孕所必需的.

科学领域:

生殖生物学生殖生物学
细胞生物学细胞生物学
植入的分子机制的植入.

背景情况:

由于植入失败导致的女性不育和早期怀孕损失是全球重要的健康问题.
热囊细胞粘附和侵入对于成功的胚胎植入至关重要,但调节机制仍然不完全理解.
氨酸18 (KRT18) 涉及早期胚胎发育,但其在植入过程中的作用尚不清楚.

研究的目的:

在胚胎植入过程中研究素18 (KRT18) 的生理功能.
阐明KRT18在植入过程中调节热囊细胞行为的分子机制.

主要方法:

在热囊细胞中对KRT18表达的定量分析.
在体内研究使用KRT18在小鼠胚胎中进行敲击.
在体外测试评估热囊细胞迁移和入侵.
微尺度热泳 (MST) 和分子生物学技术来确定KRT18-E-cadherin相互作用.

主要成果:

在 trofhoblast 细胞中,KRT18 的表达很高.
在小鼠胚胎中,KRT18的淘汰显著抑制了胚胎的粘附和植入.
在体外,抑制KRT18损害了 trofhoblast 细胞迁移和入侵.
发现KRT18在热囊细胞中直接结合和稳定细胞表面E-cadherin.

结论:

在胚胎植入过程中,KRT18在调节热囊细胞入侵和粘附方面发挥着至关重要的作用.
该机制涉及KRT18直接与热囊细胞表面的E-cadherin相互作用和稳定.
这些发现为成功胚胎植入的分子基础和不孕症治疗的潜在目标提供了新的见解.

关键词:

细胞入侵细胞入侵细胞迁移细胞迁移这是E-cadherin.胚胎的粘附胚胎的粘附胚胎植入胚胎的植入方式在KRT18中,KRT18是KRT18.

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