在帕金森病原和诊断中携带α-Synuclein的星系细胞外囊泡
Pan Wang1,2, Guoyu Lan3, Bin Xu1
1Department of Pathology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310002, China.
Translational neurodegeneration
|August 24, 2023
概括
在星球细胞中的α-syn核素 (α-syn) 积累增加了来自星球细胞的细胞外囊泡 (AEV). 血液中的这些携带α-syn的AEV显示出作为帕金森病生物标志物的潜力.
科学领域:
- 神经科学是一个神经科学.
- 生物化学 生物化学
- 细胞生物学 细胞生物学
背景情况:
- 阿尔法-同核素 (α-syn) 积累是帕金森病 (PD) 发病的核心原因,它发生在神经元和神经元中,就像天体细胞一样.
- 控制星细胞α-syn水平和聚合的机制尚未完全理解.
- 细胞外囊泡 (EVs) 调解α-syn扩散,但它们在PD天体细胞中的作用尚不清楚,尽管血液中的中枢神经系统衍生的EVs被探索为生物标志物.
研究的目的:
- 为了研究星体细胞外囊泡 (AEVs) 在帕金森病 (PD) 中的作用.
- 为了确定α-syn沉积是否影响AEV的生产和特征.
- 评估对PD的外周血液中α-syn载体AEVs的诊断潜力.
主要方法:
- 主要星体细胞被用α-syn (A53T等离子体或聚合物) 来操纵.
- 量化了由天体细胞衍生的EVs (AEVs) (纳米粒子跟踪,免疫光).
- 评估了溶解体功能;在PD,MSA和HC队列中测量了血AEV (GLT-1+).
主要成果:
- α-syn沉积显著增加了星球细胞中的AEV产量,这与溶酶体功能障碍有关.
- 与健康对照组 (HC) 和多个系统缩 (MSA) 患者相比,在PD患者中,α-syn载体AEV显著升高.
- 使用AEV和α-syn水平的综合模型实现了高诊断准确性 (AUC>0.87),用于区分PD.
结论:
- 病理性α-syn沉积,可能是通过 lysosomal 功能障碍,增强了天体细胞 EV 分泌.
- 血液中含有α-syn的AEVs是PD诊断和差异诊断的有希望的生物标志物.
更多相关视频
12:01Detection of Disease-associated α-synuclein by Enhanced ELISA in the Brain of Transgenic Mice Overexpressing Human A53T Mutated α-synuclein
Published on: May 30, 2015
10.8K
14:45Analyzing the Parkinson's Disease Mouse Model Induced by Adeno-associated Viral Vectors Encoding Human α-Synuclein
Published on: July 29, 2022
6.0K
相关概念视频
01:22Lysosomal Hydrolases
3.8K
Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...
3.8K
01:15Parkinson's Disease: Overview
596
Neurodegenerative disorders are progressive diseases that cause irreversible damage and loss to neurons in specific brain areas. Examples of these disorders include Parkinson's disease, Alzheimer's disease, Multiple Sclerosis (MS), and Amyotrophic Lateral Sclerosis (ALS). These disorders share characteristics such as proteinopathies, selective neuronal vulnerability, and a complex interplay between genetic and environmental factors. The primary therapeutic goal for these conditions is...
596
01:37Neural Regulation
39.5K
Digestion begins with a cephalic phase that prepares the digestive system to receive food. When our brain processes visual or olfactory information about food, it triggers impulses in the cranial nerves innervating the salivary glands and stomach to prepare for food.
39.5K
03:03Amyloid Fibrils
9.6K
Amyloid fibrils are aggregates of misfolded proteins. Under most circumstances, misfolded proteins are either refolded by chaperone proteins or degraded by the proteasome. However, in the case of a mutation or a disease, these proteins can accumulate to form large clusters and often further assemble to form elongated fibers, called fibrils.
Amyloid deposits were observed as early as 1639 in the liver and the spleen. In 1854, Rudolph Virchow performed iodine staining,...
Amyloid deposits were observed as early as 1639 in the liver and the spleen. In 1854, Rudolph Virchow performed iodine staining,...
9.6K