鼻腔泄漏:在成年人中检测呼吸道病原体的独特增强替代方案
Elandri Fourie1, Eva S Bording2, Sjoerd M Euser3
1Spaarne Gasthuis Academy, Spaarne Gasthuis, Hoofddorp, The Netherlands.
Journal of medical virology
|August 25, 2023
概括
鼻拭子 (RS) 能够有效地检测到82%的灵敏度的呼吸道病原体,为结合鼻和口 (NP/OP) 拭子提供了更舒适的替代方案. 这种鼻腔排泄方法为病原体检测提供了可靠的结果.
科学领域:
- 传染性疾病 传染性疾病
- 微生物学 微生物学
- 诊断技术 诊断技术 诊断技术
背景情况:
- 上呼吸道感染 (URTI) 在全球造成重大健康负担.
- 目前用于呼吸道病原体的分子检测方法通常是侵入性的和不舒服的.
- 需要更多的患者友好的诊断方法.
研究的目的:
- 为了评估鼻抽样 (RS) 的诊断能力和耐受性,用于检测呼吸道病原体.
- 为了比较RS对结合鼻和口 (NP/OP) 拭子的疗效.
- 评估鼻水作为病原体鉴定样本的可靠性.
主要方法:
- 一项涉及100名成年人在SARS-CoV-2检测设施的比较研究.
- 从患有鼻水的参与者那里收集NP/OP和RS两种抽样.
- 使用SARS-CoV-2聚合酶链反应 (PCR) 和多重结依赖探头放大 (MLPA) 检测呼吸道病原体PCR.
- 使用视觉模拟尺度 (VAS) 评分和问卷评估拭子耐受性的评估.
主要成果:
- 组合的NP/OP抽样检测到104种病原体,而RS检测到83种病原体.
- 总的来说,在89名成年人中发现了108种呼吸道病原体.
- 与NP/OP抽样相比,RS显示了82%的灵敏度和100%的特异性.
- RS比NP/OPswabs更容易承受 (p < 0.001). RS比NP/OPswabs更容易承受 (p < 0.001). RS比NP/OPswabs更容易承受 (p < 0.001). RS比NP/OPswabs更容易承受 (p < 0.001).
结论:
- 通过鼻拭子收集的鼻分泌物是检测呼吸道病原体的可靠媒介.
- RS为检测病原体提供了一种更耐受的替代方法,而不是侵入性NP/OP扫描.
- 这种方法有望在呼吸道诊断中改善患者的舒适度.
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