多功能金纳米颗粒的分子表面量化使用紫外线可见吸收光谱法解卷法.
Jordan C Potts1, Akhil Jain2, David B Amabilino3
1Division of Advanced Materials and Healthcare Technologies, School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, U.K.
Analytical chemistry
|August 25, 2023
概括
在多功能金纳米粒子 (AuNPs) 上量化表面分子对于它们的应用至关重要. 这项研究引入了一种新的UV-Vis光谱法,用于准确测量AuNPs上结合的甲和甲.
科学领域:
- 纳米技术 纳米技术
- 生物化学 生化学
- 频谱学是一种光谱学.
背景情况:
- 多功能黄金纳米粒子 (AuNPs) 在传感,成像,药物输送和医学方面表现有前途.
- 精确量化表面结合分子对于确定AuNPs的生物活性至关重要.
- 现有的方法缺乏精度来量化多个表面结合分子在多功能AuNPs.
研究的目的:
- 开发和验证一种精确的方法来量化多功能AuNP上的表面结合分子.
- 确定与单个AuNP结合的细胞染色体C (Cyt C) 和甲 (Zn Porph) 的度和数量.
- 为了评估样品净化指南的未结合分子的存在.
主要方法:
- 利用解卷和莱文伯格-马奎特算法对紫外线可见吸收光谱进行适配.
- 采用动态光散射 (DLS) 和泽塔电位测量用于功能化确认.
- 使用传输电子显微镜 (TEM) 与光谱和DLS一起验证AuNP大小并防止聚合.
主要成果:
- 尽管吸收带重叠,但成功量化了与AuNPs结合的Cyt C和Zn Porph的精确度.
- 确定了每 AuNP 附着的 Cyt C 和 Zn Porph 分子的数量.
- 识别了未结合的分子,表明可能需要加强净化.
结论:
- 开发的UV-Vis光谱解卷方法可以准确量化多功能AuNP上的表面结合分子.
- 这种技术为优化基于AuNP的药物递送系统和生物传感器提供了宝贵的见解.
- 该方法简单,有效,有助于评估AuNP功能化的样本纯度.
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