饮食中的蛋白质水平通过从哺乳母羊中获得的便样本来调节肠道微生物组合
Jiachong Liang1, Sikandar Ali1,2, Chunrong Lv1
1The Small Ruminant Department, Yunnan Animal Science and Veterinary Institute, Kunming, Yunnan, China.
Frontiers in endocrinology
|August 25, 2023
概括
饮食中的蛋白质水平显著改变了哺乳母羊的便细菌和基因表达. 更高的蛋白质摄入量丰富了特定的细菌和代谢基因,影响了营养利用和整体健康.
科学领域:
- 动物营养和微生物学
- 肠道微生物组分析
- 转基因组学和功能性基因分析
背景情况:
- 动物蛋白质的消化受胃肠道细菌群的影响.
- 饮食中的蛋白质水平对于动物健康和生产力至关重要,尤其是在哺乳期.
研究的目的:
- 为了表征便细菌群落及其功能基因,哺乳母羊在养不同饮食蛋白质水平时.
- 研究食蛋白对母羊微生物组成和代谢途径的影响.
主要方法:
- 16S rDNA测序和羊的便样本的基因组分析.
- 给不同组的不同人群提供了三种饮食蛋白质水平 (8.58%,10.34%,13.93%).
- 生物信息分析用于识别细菌分类,基因丰富性和代谢途径.
主要成果:
- 在所有群体中,Firmicutes和Bacteroidetes都是主要的类.
- 较高的蛋白质摄入量 (H_h组) 改变了特定细菌类的丰富性 (例如,蛋白质细菌减少,Lentisphaerae增加) 和属 (例如,Prevotella减少,Ruminococcus增加).
- 甲基因组分析显示,H_h组中涉及新陈代谢 (碳水化合物,氨基酸,核酸,维生素) 的微生物基因显著丰富.
结论:
- 饮食中的蛋白质水平动态地影响便细菌组成和哺乳母羊的功能基因表达.
- 较高蛋白质的饮食促进了微生物群落的增强代谢能力,有可能优化营养利用.
- 了解这些微生物的变化可以为改善母羊和羊羔健康和生长的饮食策略提供信息.
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