相关实验视频

Updated: Jul 18, 2025

Mouse Model of Oleic Acid-Induced Acute Respiratory Distress Syndrome

Mouse Model of Oleic Acid-Induced Acute Respiratory Distress Syndrome

Published on: June 2, 2022

miR-346通过调节肺微血管内皮细胞的功能来调节ARDS的发展

Jing Jiang¹, Fei Guo², Wei Li¹

¹Department of Pulmonary and Critical Care Medicine, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong, 264000, China.

Non-coding RNA research

|August 25, 2023

概括

微RNA-346 (miR-346) 通过促进肺炎和细胞亡,加剧了急性呼吸困扰综合征 (ARDS). 抑制miR-346可以缓解肺损伤,这表明它是ARDS的治疗目标.

科学领域:

分子生物学分子生物学
呼吸系统医学呼吸系统医学
病变发生学研究病变发生学研究

背景情况:

微RNAs (miRNAs) 越来越多地被认可为它们在疾病发展中的作用.
在急性呼吸窘迫综合征 (ARDS) 中miR-346的具体参与尚未完全阐明.
了解miRNA机制对于开发ARDS新型治疗策略至关重要.

研究的目的:

调查miR-346在ARDS病变发生过程中的作用和潜在机制.
为了确定miR-346表达水平和ARDS严重程度之间的关系.
为了确定涉及ARDS的miR-346的潜在分子标.

主要方法:

建立了一种由脂聚糖 (LPS) 诱导的ARDS小鼠模型.
定量逆转录聚合酶链反应 (RT-qPCR) 用于测量miR-346的表达.
在体内和体外 (HPMECs) 使用miR-346抑制剂抑制miR-346.
生物信息预测和miR-346目标的实验验证 (BCL6).

主要成果:

在ARDS小鼠的肺部,miR-346表达显著上调,与氧化指数负相关.
抑制miR-346减少了肺损伤标志物 (肺指数,肺水含量,NO),减轻了炎症,并减少了亡.
确定BCL6是miR-346的标;同时抑制miR-346和BCL6加剧了肺损伤和HPMEC功能障碍.

结论:

miR-346在ARDS病变发生过程中起着促炎和促的作用.
miR-346通过BCL6调节抑制HPMEC迁移,从而促进ARDS,导致亡和炎症的增加.
向miR-346为缓解ARDS提供了一个潜在的治疗策略.

关键词:

在 ARDS 中,ARDS 是指 ARDS 的类型.细胞灭亡 (apoptosis) 是一种死亡的过程.机理机制机理机制核武器的扩散在 miR-34646 里面.

更多相关视频

Author Spotlight: Advances in Quantifying Microvascular Density in Aging Murine Lungs

Author Spotlight: Advances in Quantifying Microvascular Density in Aging Murine Lungs

Published on: January 3, 2025

Implantation of Fibrin Gel on Mouse Lung to Study Lung-specific Angiogenesis

Implantation of Fibrin Gel on Mouse Lung to Study Lung-specific Angiogenesis

Published on: December 21, 2014

相关概念视频

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...