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一个双响应的NIR光探测器,用于单独和连续地识别H₂S和Cys与不同的光信号及其应用

Lisha Yue¹, Yin Ai¹, Gang Liu¹

¹Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, PR China. dinghaichang@126.com.

|August 25, 2023

概括

一种新的双位点光探针Cy-NP被开发用于对硫化 (H2S) 和氨酸 (Cys) 的敏感检测. 这种探测器使生物样本中这些重要生物分子的独立和持续监测成为可能.

科学领域:

化学生物学化学生物学
生物医学工程生物医学工程
分析化学分析化学

背景情况:

硫化 (H2S) 和氨酸 (Cys) 是具有重要的生理作用的关键生物醇.
开发选择性和敏感的探针,同时检测H2S和Cys,对于理解它们的生物功能至关重要.
现有的检测方法可能缺乏特异性或同时监测两种分析物的能力.

研究的目的:

合成和描述一种能够检测H2S和Cys.的新型双位点光探测器 (Cy-NP).
评估Cy-NP探针的灵敏度,选择性和机制.
展示Cy-NP在复杂生物系统中用于监测H2S和Cys的实际应用.

主要方法:

合成双部位光探测器Cy-NP,包含纳夫他胺和氨酸染料部分.
光光谱测试以评估对H2S和Cys的敏感性和选择性.
高分辨率质谱 (HRMS) 和密度函数理论 (DFT) 计算以阐明反应机制.
在生肉样本和MCF-7癌细胞中应用探针进行实时监测.

主要成果:

合成的Cy-NP探头在可见和近红外区域呈现双重发射.
Cy-NP对H2S (LOD 0.15 μM) 和Cys (LOD 1.4 μM) 具有很高的灵敏度和选择性,而其他生物醇的干扰最小.
探测器的化学机制通过HRMS和DFT得到证实.
在生肉中监测H2S的成功应用以及在MCF-7细胞中同时持续检测H2S和Cys.

结论:

开发的Cy-NP探头是一个多功能和有效的工具,用于敏感和选择性检测H2S和Cys.
通过Cy-NP,可以在生物样本中独立和持续地监测这些硫醇,为生物化学研究提供了新的可能性.
这种探测器有可能在食品安全和生物医学诊断领域应用.

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