[通过工程设计大肠杆菌的一种碳模块来有效合成L-氨酸]
Bo Zhang1,2, Ying Wang1,2, Kun Niu1,2
1The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China.
Sheng wu gong cheng xue bao = Chinese journal of biotechnology
|August 25, 2023
概括
改造大肠杆菌以产生更多的L-氨酸,一种必需的氨基酸. 优化单碳模块显著提高了微生物发酵中的L-氨酸产量.
科学领域:
- 微生物生物技术 微生物生物技术
- 代谢工程是代谢工程.
- 生物化学工程是生物化学工程.
背景情况:
- 甲氨酸是一种对人类健康至关重要的必需氨基酸,在动物料,医药和食品工业中具有广泛的应用.
- 高效的微生物生产L-氨酸对于满足工业需求至关重要.
- 之前的工作建立了一个工程 * Escherichia coli * M2 菌株 (W3110 ΔIJAHFEBC/PAM) 作为进一步优化的基础.
研究的目的:
- 使用模块化代谢工程构建一个高收益的L-氨酸生产菌株.
- 增强单碳模块和前体供应途径,以改善L-氨酸生物合成.
- 验证代谢工程策略在增加L-氨酸产量的有效性.
主要方法:
- 过度表达的甲基二甲基四基叶酸还原酶 (MetF) 和选的基甲基转移酶 (GlyA),以优化单碳模块.
- 过度表达的氨酸酶 (MalY) 和氨酸转运基因 (fliY) 来增强前体供应 (L-homocysteine和L-cysteine).
- 进行了摇瓶和5L发酵器实验,以评估L-氨酸的生产.
主要成果:
- 优化单碳模块和前体路径使摇瓶中的L-氨酸产量从2.8g/L增加到4.05g/L.
- 在5L发酵器中达到18.26g/L的最大L-甲氨酸产量.
- 证明了单碳模块对L-氨酸生物合成的重大影响.
结论:
- 模块化代谢工程,特别是优化单碳模块,是增强大肠杆菌L-氨酸生产的有效策略.
- 这种工程菌株显示出在微生物发酵中进一步改善L-氨酸的潜力.
- 这项研究为推进工业微生物生产L-氨酸提供了基础.
更多相关视频
11:51Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
Published on: April 27, 2018
12.0K
16:11Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology
Published on: September 16, 2013
64.1K
相关概念视频
Amino Acid Biosynthetic Pathways
36
Amino acid biosynthesis is essential for cell growth, protein synthesis, and metabolic regulation. Cells generate essential and non-essential amino acids from metabolic intermediates to sustain vital biological functions. These intermediates originate from key metabolic pathways: glycolysis, the tricarboxylic acid (TCA) cycle, and the pentose phosphate pathway. Important precursors include α-ketoglutarate, pyruvate, oxaloacetate, phosphoenolpyruvate, and erythrose-4-phosphate, which...
36
Biosynthesis in Bacteria
39
Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...
39
Coordination of Gene Expression Processes in Bacteria
40
The DNA replication, transcription, and translation processes are intricately coupled in bacteria, allowing efficient gene expression and rapid protein synthesis. While this physical and functional coordination is advantageous, it introduces challenges that bacteria overcome through specific regulatory mechanisms.Coupling of Replication, Transcription, and TranslationThe coupling of replication, transcription, and translation is a hallmark of bacterial gene expression. As the replisome unwinds...
40
