[使用载体来增强Bacillus coagulans DSM1的酸耐受性]
Jing Li1,2, Yu Wang1,2, Bo Yu2
1College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, Hebei, China.
Sheng wu gong cheng xue bao = Chinese journal of biotechnology
|August 25, 2023
概括
提高Bacillus coagulans DSM1中的乳酸耐受性是高效聚乳酸生产的关键. 这项研究确定了增强酸性耐受性的特定基因,有助于开发出更好的乳酸生产者.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 代谢工程是代谢工程.
背景情况:
- 光学纯净的l-乳酸是聚乳酸 (PLA) 生产的关键前体.
- 高度的乳酸抑制生产微生物的生长,需要提高耐酸性.
- 细菌凝血菌DSM1是l-乳酸的重要生产者,但其耐酸性需要提高.
研究的目的:
- 为了研究载体在乳酸发酵过程中对DSM1凝血菌的酸性耐受性中的作用.
- 为了确定参与增强B. coagulans乳酸耐受性的基因和途径.
主要方法:
- 使用比较转录基因分析来识别差异表达的基因.
- 实时PCR被用来验证关键基因的转录水平.
- 进行基因操纵 (过度表达) 来评估已识别的基因的功能影响.
主要成果:
- 一些基因,包括RS06895,RS10595,RS00500和RS10635,在乳酸发酵过程中显示出显著的上调调节.
- 过度表达RS10595导致pH值为6.0的生长和产量减少,但在4.6的更酸的pH值增强了产量.
- 转录组数据提供了关于B. coagulans.酸耐受性潜在机制的见解.
结论:
- 该研究确定了特定的转运基因,这些基因在B.coagulans DSM1.1.的酸耐受性中发挥作用.
- 了解这些机制可以指导开发强大的微生物菌株,以有效地生产l-乳酸和PLA.
- 对这些基因的进一步研究可能会促进对优质乳酸生产微生物的工程.
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