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Rin Yamada1,2, Koji Ohnishi3, Cheng Pan1
1Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto, Japan.
Microbiology and immunology
|August 25, 2023
概括
人类鼻巨细胞 (SMs) 表达与树突细胞 (DC) 相关的分子CD209,并表现出不同的CD169反应性,这表明它们可能是寄居巨细胞. 这澄清了抗癌免疫中的SM分子概况.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 癌症研究 癌症研究
背景情况:
- 阴道巨细胞 (SMs) 在抗癌免疫反应中发挥作用,但它们在人类淋巴结 (LNs) 中的分子特征尚未完全理解.
- 澄清SMs的分子形状对于理解它们在免疫系统中的功能和潜在的治疗应用至关重要.
研究的目的:
- 为了识别树突细胞 (DC) 或巨细胞相关的分子,表达在人体SMs内LNs.
- 研究CD169抗体的不同反应性和干扰素-α (IFN-α) 刺激对SMs的影响.
主要方法:
- 免疫组织化学使用对Iba-1,CD163,CD169和CD209的抗体对人类LN段进行检测.
- 分析CD169抗体活性 (克隆SP216和HSn 7D2) 以及其通过IFN-α刺激的调制.
- 使用小干扰RNA (siRNA) 对CD169.9进行验证.
- RT-qPCR和光在位杂交 (FISH) 检测CCL8和CXCL10mRNA表达.
- 对LNs单细胞RNA测序数据的分析.
主要成果:
- 人类LN中的SMs对Iba-1,CD163,CD169和CD209具有强烈阳性.
- CD169克隆SP216与大多数SMs (细胞表面) 反应,而克隆HSn 7D2与亚群 (细胞质) 反应,在IFN-α刺激后反应性增加.
- 在培养的巨细胞中,IFN-α诱导了CCL8和CXCL10mRNA,但通过FISH在SM中没有检测到这些.
- 单细胞RNA测序表明单细胞表达CXCL10基因的最高水平.
结论:
- 人类SMs表达CD209,一个DC相关的分子.
- CD169反应的异质性可能是由于IFN刺激的蛋白质修饰.
- 在SM中没有可检测的CXCL10mRNA,这表明它们的功能是作为居民巨细胞.
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