使用冰再结晶抑制剂改善了人类诱导多能干细胞 (iPSC) 和iPSC衍生的神经元的冷保存

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Salma Alasmar¹, Jez Huang², Karishma Chopra¹

¹Department of Chemistry and Biomolecular Sciences, University of Ottawa, Faculty of Science, Ottawa, ON, Canada.

Stem cells (Dayton, Ohio)

|August 25, 2023

概括

冰再结晶抑制剂 (IRI),特别是2FA,增强人类诱导的多能干细胞 (iPSC) 恢复,并在冷保存后改善iPSC衍生的神经元 (iPSC-Ns) 的功能恢复,从而提高细胞治疗潜力.

科学领域:

干细胞生物学干细胞生物学
再生医学是一种再生医学.
低温生物学低温生物学

背景情况:

人类诱导的多能干细胞 (iPSC) 和它们的神经元衍生物 (iPSC-Ns) 对再生医学至关重要.
有效的冷保存对于iPSC-N在细胞替代疗法中的临床应用至关重要.

研究的目的:

研究冰再结晶抑制剂 (IRI) 作为iPSC和iPSC-N的新型冷保护剂.
评估IRI对细胞活力,多能性和解后功能恢复的影响.

主要方法:

测试N-aryl-D-aldonamides (2FA) 作为 iPSC 和 iPSC-Ns 的 IRI.
评估解后的生命力,多能性,神经元标记物表达和功能网络活动.
将IRI处理的细胞与传统的冷保存 (CS10) 控制器进行比较.

主要成果:

2FA显著增加了iPSC的活力和恢复,而不会影响多能性.
2FA并没有显著改善iPSC-N的活力,但加速了神经元网络活动和突触功能的恢复.
2FA冷保存的iPSC-N保持了神经元标记物和对神经活性化合物的功能反应.

结论:

使用IRI优化冷保存介质是iPSC和iPSC-N的功能冷保存的一个有希望的策略.
用IRI增强的冷保存通过改善关键细胞类型的功能恢复来支持基于iPSC的治疗方法的开发.

关键词:

冷保存的方法冰的再结晶冰的再结晶冰的再结晶抑制剂冰的再结晶抑制剂诱导多能干细胞的诱导干细胞.神经元神经元的神经元公司的可行性,可行性.

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