大气和室温等离子体 (ARTP) 在微生物繁殖中的应用
Qin Zhang1,2, Renyun Miao1,2, Rencai Feng1,2
1Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu 610299, China.
Current issues in molecular biology
|August 25, 2023
概括
大气和室温等离子体 (ARTP) 提供了一种高效的微生物突变发生法. 这种技术增强了稳定突变体的DNA损伤,对于微生物繁殖和生产力提升非常有价值.
科学领域:
- 微生物遗传学 微生物遗传学
- 生物技术是生物技术.
- 血科学是一门科学课.
背景情况:
- 传统的突变发生法方法可能是低效的,并且涉及危险的化学物质.
- 需要先进的技术来产生稳定的微生物突变.
- 微生物育种对于提高生产力和所需特征至关重要.
研究的目的:
- 突出大气和室温等离子体 (ARTP) 作为微生物突变发生器工具的潜力.
- 为了证明ARTP在诱导DNA损伤和创建稳定的突变菌株方面的有效性.
- 探索ARTP在细菌,真菌和微藻繁殖中的应用.
主要方法:
- 使用大气和室温等离子体 (ARTP) 进行微生物突变.
- 使用等离子技术诱导微生物细胞中的DNA损伤.
- 产生和表征稳定的突变菌株.
主要成果:
- 在微生物中,ARTP有效诱导DNA损伤.
- 产生了具有改善特性的稳定突变菌株.
- 与传统方法相比,ARTP具有成本效益,并避免使用危险化学品.
结论:
- 大气和室温等离子体 (ARTP) 是微生物繁殖的一个有前途的技术.
- ARTP促进了突变菌株的发展,提高了产量和质量.
- 持续优化ARTP将推动微生物育种方面的创新.
相关概念视频
Physical Methods for Controlling Microbial Growth: Radiation and Filtration
54
Radiation and filtration are essential tools for microbial control, targeting microorganisms through distinct mechanisms. Radiation eliminates microbes by damaging their DNA, either killing them or inhibiting their growth. Based on wavelength, radiation is classified into two types: nonionizing and ionizing radiation.Non-ionizing radiation, such as UV radiation (200–400 nm), is absorbed by DNA, causing defects that effectively disinfect surfaces, air, and water, including safety cabinets.
54
Physical Methods for Controlling Microbial Growth: Temperature
49
Heat is a widely used method to control microbial growth by targeting and denaturing cellular proteins, thereby killing or inactivating microbes. This method's effectiveness is quantified using parameters such as the thermal death point (TDP), thermal death time (TDT), and decimal reduction time (D value). TDP represents the lowest temperature at which all microorganisms in a liquid suspension are eliminated within 10 minutes, whereas TDT is the time necessary to achieve sterilization at a...
49
Key Techniques in Microbiology
70
Aseptic techniques prevent contamination, ensure experimental accuracy, and protect researchers and microbial cultures. These techniques are essential in clinical, industrial, and research settings where sterility is required.Maintaining Sterility in Laboratory PracticesScientists maintain sterility by sterilizing tools with heat or chemicals, disinfecting work surfaces, and handling cultures in controlled environments. Working near an open flame or within a laminar flow hood reduces the risk...
70
Methods for Controlling Microbial Growth
69
Microbial growth control refers to various methods employed to inhibit, reduce, or eliminate microorganisms to ensure safety and hygiene across different settings. These methods are categorized based on the target environment and the level of microbial control required.Biocides are versatile agents designed to control microorganisms by either inhibiting their growth or outright killing them. These agents work through various physical, chemical, mechanical, or biological mechanisms. The...
69
Factors Influencing Microbial Growth: Temperature
67
Microorganisms display remarkable adaptations, enabling them to thrive in diverse ecological niches across a wide range of temperatures. Temperature profoundly influences microbial growth by affecting enzymatic activity, membrane fluidity, and other cellular processes.Each microorganism operates within a specific temperature range defined by three cardinal points: minimum, optimum, and maximum. Below the minimum temperature, membranes lose fluidity, halting transport processes. Above the...
67
Biological Methods for Microbial Control
62
Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
62


