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揭露Nilaparvata lugens Stål基因通过转录组分析和基因沉默来定义与大米的兼容和不兼容的相互作用

Priyadarshini Rout¹, Nihal Ravindranath¹, Dinkar Gaikwad¹

¹MS Swaminathan School of Agriculture, Centurion University of Technology and Management, Paralakhemundi 761211, Odisha, India.

Current issues in molecular biology

|August 25, 2023

概括

了解棕色植物 (BPH) 生物型4与大米的相互作用对于害虫控制至关重要. 这项研究揭示了BPH在食耐药大米品种时的关键生理和基因表达变化,确定了生存至关重要的基因.

科学领域:

农业昆虫学农业昆虫学
分子生物学分子生物学
植物与昆虫的相互作用

背景情况:

棕色植物 (Nilaparvata lugens,BPH) 是一种重要的水害虫,其多种生物型使控制策略复杂化.
有限的研究存在于米-BPH相互作用的分子机制,具体到印度次大陆的BPH生物型4.

研究的目的:

研究BPH生物型4在与敏感 (TN1) 和耐药 (PTB33) 米品种相互作用时的转录组,生理和基因沉默反应.
为了确定关键的基因和途径参与BPH适应和存活在米-BPH相互作用期间.

主要方法:

对BPH生物型4转录组,生理学 (蜂蜜产量,体重增加,糖含量) 和基因表达的比较分析.
用RNA干扰 (RNAi) 来使特定的BPH基因 (NlCP1,NlCYP320a1,NlTret1) 沉默,以评估它们的功能.

主要成果:

不兼容的相互作用 (耐药大米) 导致BPH的蜂蜜露减少,体重增加为负数,三糖增加,葡萄糖降低.
转录组分析确定了1875个与皮肤皮的发育,糖代谢,排毒和变相关的差异性表达基因.
抑制NlCP1,NlCYP320a1和NlTret1显著影响了BPH生理学和生存率.

结论:

乙PH生物型4对抗性大米表现出明显的生理和分子反应,突出代谢和发育调整.
鉴定出来的基因对BPH的生存至关重要,并为开发针对这种主要的水害虫的新害虫管理策略提供了潜在的目标.

关键词:

它们是RNAiRNAi.棕色的植物跳棕色的植物跳兼容和不兼容的相互作用.米米饭米饭米饭米饭.转录组 (transcriptome) 是一个转录组.

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