在化疗诱导的外周神经病变中识别circRNA-miRNA-mRNA调节网络
Fei Cao1, Xintong Wang1, Qingqing Ye1
1Department of Anesthesiology, State Key Laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, Guangzhou 510060, China.
Current issues in molecular biology
|August 25, 2023
概括
循环RNAs (circRNAs) 在化疗诱导的外围神经病变 (CIPN) 中起着至关重要的作用. 这项研究确定了参与CIPN发展的关键circRNA和免疫细胞相互作用.
科学领域:
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
- 基因组学就是基因组学.
背景情况:
- 化疗诱导的周围神经病变 (CIPN) 是癌症治疗的显著不良影响.
- 在CIPN背后的分子机制,特别是循环RNAs (circRNAs) 的作用,尚不清楚.
研究的目的:
- 为了研究circRNAs和CIPN之间的关联.
- 为了确定关键的circRNAs,microRNAs (miRNAs) 和信使RNAs (mRNAs) 参与CIPN病变.
- 探索免疫微环境在CIPN中的作用.
主要方法:
- 使用Taxol.net构建CIPN模型
- RNA测序用于识别差异表达的circRNA,长非编码RNA和mRNA.
- 对circRNA-miRNA-mRNA网络的生物信息预测.
- 功能丰富和免疫透分析.
- 使用RT-qPCR验证枢纽基因.
主要成果:
- 在CIPN模型中确定了134个差异表达的circRNA,353个lncRNA和86个mRNA.
- 已建立的circRNA-miRNA-mRNA网络包括15个circRNA,18个miRNA和11个mRNA.
- 在免疫路径中发现了差异表达的mRNA的丰富,特别是涉及T细胞,单细胞和巨细胞.
- 鉴定并验证了五个枢纽基因 (Cdh1,Satb2,Fas,P2ry2,Zfhx2) 与CIPN中的特定免疫细胞相关.
- 预测相关疾病,转录因子和枢纽基因的潜在药物.
结论:
- circRNAs在CIPN.的发展中发挥着关键作用.
- 免疫微环境,包括T细胞和巨细胞,在CIPN.中起着重要作用.
- 这项研究为CIPN的分子机制和潜在的治疗点提供了新的见解.
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